Euphorbia bivonae 提取物的植物化学成分及其对卤虫致死率和胚胎肾（HEK293）细胞的细胞毒性作用。
Phytochemical Compounds of Euphorbia bivonae Extract and Their Cytotoxicity Effects on the Lethality of Brine Shrimp Artemia salina and Embryonic Kidney (HEK293) Cells.
机构信息
Laboratory of Dryland and Oasis Cropping, Institute of Arid Zone of Médenine, University of Gabes, ElFjè, Medenine 4119, Tunisia.
Laboratory of Biodiversity and Aquatic Ecosystems UR/11ES72, Ecology and Planktonology, Department of Life Sciences, University of Sfax, Soukra Road Km 3.5, BP1171, 3000, Sfax, Tunisia.
出版信息
Chem Biodivers. 2023 May;20(5):e202201135. doi: 10.1002/cbdv.202201135. Epub 2023 May 3.
In this research article, we investigated the effect of Euphorbia bivonae extract compounds on the lethality of brine shrimp Artemia salina and on embryonic cell lines (HEK293) proliferation. Our GC/MS analysis revealed that the E. bivonae ethanolic extract contained essentially sitosterol, euphol, and lupeol. The 24-h LC50 was determined using the probit analysis method (LC50=357.11 mg l ). Depending on this cytotoxicity test result, E. bivona extract induced a significant increase in Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-Peroxidase (GPx) activities, and lipid peroxidation (LPO) in A. salina larvae. In addition, the cytotoxicity effect of this extract had proved against the HEK293 cell lines in vitro. We suggest that the three compounds of E. bivonae extract (sitosterol, euphol, and lupeol) are the most responsible for this cytotoxicity. The possible application of this extract as an alternative natural antiproliferative is considered.
在这项研究中，我们研究了大戟属双叶提取物化合物对卤虫致死率和胚胎细胞系（HEK293）增殖的影响。我们的 GC/MS 分析表明，大戟属双叶乙醇提取物主要含有豆甾醇、 euphol 和 lupeol。采用概率单位分析方法（LC50=357.11 mg/L）测定 24 小时 LC50。根据这项细胞毒性试验结果，大戟属双叶提取物诱导卤虫幼虫中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活性和脂质过氧化（LPO）显著增加。此外，该提取物的细胞毒性作用已被证明对体外的 HEK293 细胞系有效。我们认为，大戟属双叶提取物中的三种化合物（豆甾醇、 euphol 和 lupeol）是导致这种细胞毒性的主要原因。考虑将该提取物作为一种替代的天然抗增殖剂进行应用。
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