同时对时间分辨荧光发射光谱进行光谱时间建模。

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum.

出版信息

IEEE Trans Biomed Eng. 2023 Aug;70(8):2395-2403. doi: 10.1109/TBME.2023.3244664. Epub 2023 Jul 18.

DOI:10.1109/TBME.2023.3244664

Abstract

Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

摘要

互补金属氧化物半导体 (CMOS) 单光子雪崩二极管 (SPAD) 技术的创新推动了新一代基于点的时间分辨荧光光谱 (TRFS) 仪器的发展。这些仪器提供了数百个光谱通道，可在高光谱和时间分辨率下在宽光谱范围内收集荧光强度和荧光寿命信息。我们提出了 Multichannel Fluorescence Lifetime Estimation，即 MuFLE，这是一种有效的计算方法，可利用独特的多通道光谱数据，重点是同时估计发射光谱和各自的光谱荧光寿命。此外，我们还表明，该方法可以从混合样品中估计荧光团的个体光谱特性。