College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, Shaanxi, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
Bioresour Technol. 2023 Jul;379:129025. doi: 10.1016/j.biortech.2023.129025. Epub 2023 Apr 5.
The production of xylo-oligosaccharides (XOS) from lignocelluloses with conjugated acid-base system is highly efficient. However, XOS production from wheat straw with conjugated system of acetic acid/sodium acetate (HAc/NaAc) has not been reported. In addition, the impact of delignification of wheat straw for XOS production was not clear. In this study, the optimal conditions for HAc/NaAc hydrolysis were: 0.4 M, molar ratio 10:1, 170 °C, and 60 min. The yield of XOS could be rose to 50.2% after the HAc/NaAc hydrolysate was hydrolyzed using xylanase. After 70.3% of lignin in wheat straw was removed by hydrogen peroxide-acetic acid treatment, the XOS yield of delignified wheat straw using HAc/NaAc reached 54.7%. Finally, a glucose yield of 96.6% was gained by cellulase from wheat straw solid. This work suggested that HAc/NaAc hydrolysis could efficiently produce XOS from wheat straw and the delignification of wheat straw was conducive to XOS and monosaccharides production.
采用酸-碱共轭体系从木质纤维素生产木二糖（XOS）具有很高的效率。然而，利用乙酸/醋酸钠（HAc/NaAc）共轭体系从麦草生产 XOS 尚未见报道。此外，麦草中木质素脱除对 XOS 生产的影响也不清楚。在本研究中，HAc/NaAc 水解的最佳条件为：0.4 M，摩尔比 10:1，170°C，60 min。HAc/NaAc 水解产物经木聚糖酶水解后，XOS 的产率可提高到 50.2%。经过过氧化氢-乙酸处理去除 70.3%的麦草木质素后，HAc/NaAc 对脱木质素麦草的 XOS 产率达到 54.7%。最后，从麦草固体中用纤维素酶获得了 96.6%的葡萄糖产率。这项工作表明，HAc/NaAc 水解可以从麦草中有效地生产 XOS，并且麦草的木质素脱除有利于 XOS 和单糖的生产。
