Gao Kaikai, Wang Hanxing, Chen Yu, Chu Jie, Zhang Junhua
College of Forestry, Northwest A&F University, Yangling 712100, China.
College of Forestry, Northwest A&F University, Yangling 712100, China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
Bioresour Technol. 2023 Nov;387:129597. doi: 10.1016/j.biortech.2023.129597. Epub 2023 Jul 31.
Currently, the production of xylo-oligosaccharides (XOS) from lignocelluloses by chelating system hydrolysis has not been investigated. Herein, iron (III) chloride/sodium citrate (IC/SC) chelating system hydrolysis and xylanase hydrolysis were used to produce XOS from poplar. Then, the delignification of IC/SC-hydrolyzed poplar was performed by p-toluenesulfonic acid (p-TsOH) pretreatment to increase the accessibility of cellulase. The results demonstrated that 42.3% of XOS with an extremely low by-product (xylose/XOS = 0.11) was produced from poplar by 50 mM IC/SC hydrolysis (molar ratio of 1:1, 170 °C, 60 min) and xylanase hydrolysis. The second step IC/SC hydrolysis and xylanase hydrolysis of poplar increased the yield of XOS to 51.3%. Finally, the glucose yield of p-TsOH-pretreated poplar (60% p-TsOH, 70 °C, 30 min) was greatly increased from 37.5% to 83.8% by cellulase hydrolysis with Tween 80 addition. The novel strategy proposed in this work was feasible for XOS and monosaccharides production from poplar.
目前，尚未研究通过螯合体系水解从木质纤维素生产低聚木糖（XOS）。在此，采用氯化铁（III）/柠檬酸钠（IC/SC）螯合体系水解和木聚糖酶水解从杨树中生产XOS。然后，通过对甲苯磺酸（p-TsOH）预处理对IC/SC水解后的杨树进行脱木质素处理，以提高纤维素酶的可及性。结果表明，通过50 mM IC/SC水解（摩尔比1:1，170°C，60分钟）和木聚糖酶水解，从杨树中生产出了42.3%的XOS，副产物极低（木糖/XOS = 0.11）。杨树的第二步IC/SC水解和木聚糖酶水解使XOS的产率提高到了51.3%。最后，通过添加吐温80进行纤维素酶水解，对甲苯磺酸预处理后的杨树（60% p-TsOH，70°C，30分钟）的葡萄糖产率从37.5%大幅提高到了83.8%。本工作中提出的新策略对于从杨树生产XOS和单糖是可行的。
