Department of Biotechnology, Shivaji University, Kolhapur, 416004, Maharashtra, India.
Department of Biotechnology, Shivaji University, Kolhapur, 416004, Maharashtra, India; Department of Biochemistry, Savitribai Phule Pune University, Pune, 411007 Maharashtra, India.
Environ Pollut. 2023 Jun 15;327:121583. doi: 10.1016/j.envpol.2023.121583. Epub 2023 Apr 5.
Emerging contaminants removals like dyes and heavy metals from the textile effluent have an immense challenge. The present study focuses on the biotransformation and detoxification of dyes and in situ textile effluent treatment by plants and microbes efficiently. A mixed consortium of perennial herbaceous plant Canna indica and fungi Saccharomyces cerevisiae showed decolorization of di-azo dye Congo red (CR, 100 mg/L) up to 97% within 72 h. Root tissues and Saccharomyces cerevisiae cells revealed induction of various dye-degrading oxidoreductase enzymes such as lignin peroxidase, laccase, veratryl alcohol oxidase and azo reductase during CR decolorization. Chlorophyll a, Chlorophyll b and carotenoid pigments were notably elevated in the leaves of a plant during the treatment. Phytotransformation of CR into its metabolic constituents was detected by using several analytical techniques, including FTIR, HPLC, and GC-MS and its non-toxic nature was confirmed by cyto-toxicological evaluation on Allium cepa and on freshwater bivalves. Mix consortium of plant Canna indica and fungi Saccharomyces cerevisiae efficiently treated textile wastewater (500 L) and reduced ADMI, COD, BOD, TSS and TDS (74, 68, 68, 78, and 66%) within 96 h. In situ textile wastewater treatment for in furrows constructed and planted with Canna indica, Saccharomyces cerevisiae and consortium-CS within 4 days reveals reduced ADMI, COD, BOD, TDS and TSS (74, 73, 75, 78, and 77%). Comprehensive observations recommend this is an intelligent tactic to exploit this consortium in the furrows for textile wastewater treatment.
从纺织废水中去除新兴污染物，如染料和重金属，是一个巨大的挑战。本研究主要关注植物和微生物对染料的生物转化和解毒作用，以及原位纺织废水处理。多年生草本植物美人蕉和真菌酿酒酵母的混合菌丛在 72 小时内将二偶氮染料刚果红（CR，100mg/L）的脱色率提高到 97%。在 CR 脱色过程中，根组织和酿酒酵母细胞显示出各种染料降解氧化还原酶的诱导，如木质素过氧化物酶、漆酶、藜芦醇氧化酶和偶氮还原酶。在处理过程中，植物叶片中的叶绿素 a、叶绿素 b 和类胡萝卜素色素显著增加。利用傅里叶变换红外光谱（FTIR）、高效液相色谱（HPLC）和气相色谱-质谱联用（GC-MS）等多种分析技术检测到 CR 向其代谢产物的植物转化，并通过对洋葱和淡水双壳类动物的细胞毒性评估证实其无毒性质。美人蕉和酿酒酵母混合菌丛能有效处理 500L 纺织废水，在 96 小时内将 ADMI、COD、BOD、TSS 和 TDS 分别降低 74%、68%、68%、78%和 66%。在种植美人蕉、酿酒酵母和 CS 菌丛的沟内原位处理纺织废水，4 天内可降低 ADMI、COD、BOD、TDS 和 TSS（74%、73%、75%、78%和 77%）。综合观察结果表明，这是一种利用该菌丛在沟内处理纺织废水的智能策略。
