抗菌光动力疗法：烧伤患者创面感染治疗的现代技术。
Antimicrobial photodynamic therapy: modern technology in the treatment of wound infections in patients with burns.
机构信息
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Wound Care. 2023 Apr 1;32(Sup4a):xxxi-xxxviii. doi: 10.12968/jowc.2023.32.Sup4a.xxxi.
BACKGROUND
is one of the most frequently isolated microorganisms from burn wounds. Antimicrobial photodynamic therapy (aPDT) is a new strategy that may improve antimicrobial treatment.
METHOD
This study evaluated three meticillin-resistant (MRSA) and three meticillin-sensitive (MSSA) clinical isolates, which produced a biofilm with 0.1mg/ml Toluidine Blue O (TBO) (Sigma-Aldrich, Germany) with an energy density of 45J/cm and 90J/cm, for MRSA and MSSA, respectively. The antibiofilm potential of aPDT with TBO was analysed using crystal violet assays and scanning electron microscopy.
RESULTS
TBO-aPDT significantly degraded the biofilm formed by MRSA and MSSA clinical isolates (p<0.05).
CONCLUSION
Our results indicated that aPDT is an effective approach to combat bacterial biofilms associated with burn wound infection. aPDT could provide a supplemental to the treatment of wound and tissue infection, and patients with burns may benefit from combined treatments.
背景
是最常从烧伤创面分离出来的微生物之一。光动力抗菌疗法（aPDT）是一种新的抗菌治疗策略。
方法
本研究评估了三种耐甲氧西林金黄色葡萄球菌（MRSA）和三种耐甲氧西林金黄色葡萄球菌（MSSA）临床分离株，它们分别用 0.1mg/ml 甲苯胺蓝 O（TBO）（西格玛奥德里奇，德国）产生生物膜，能量密度分别为 45J/cm 和 90J/cm，用于 MRSA 和 MSSA。使用结晶紫分析和扫描电子显微镜分析 TBO-aPDT 的抗生物膜潜力。
结果
TBO-aPDT 显著降解了 MRSA 和 MSSA 临床分离株形成的生物膜（p<0.05）。
结论
我们的结果表明，aPDT 是一种有效对抗与烧伤创面感染相关的细菌生物膜的方法。aPDT 可以为创面和组织感染的治疗提供补充，烧伤患者可能受益于联合治疗。
Zotero 插件