Isakson P C, Honegger J L, Kinsky S C
J Immunol Methods. 1979;25(1):89-96. doi: 10.1016/0022-1759(79)90169-8.
The preparation of sheep red blood cells (SRBC) which have been sensitized with the haptens azobenzenearsonate, phosphorylcholine and azophenyltrimethylammonium, and are suitable for use in a plaque-forming cell assay, is described. Each hapten is first coupled to a phenolic imidoester (methyl-p-hydroxybenzimidate) and the substituted imidoesters are employed to prepare target SRBC. Details are presented concerning synthesis and purification of the imidoesters, as well as conditions for coupling to SRBC. The hapten-SRBC prepared by this method are very stable and offer significant advantages over other procedures for coupling these haptens to red blood cells.
本文描述了用偶氮苯胂酸、磷酰胆碱和偶氮苯基三甲基铵等半抗原致敏的绵羊红细胞（SRBC）的制备方法，这些红细胞适用于空斑形成细胞试验。每种半抗原首先与一种酚基亚胺酯（甲基 - 对羟基苯亚氨酸酯）偶联，然后使用取代的亚胺酯来制备目标SRBC。文中给出了亚胺酯的合成和纯化细节，以及与SRBC偶联的条件。通过这种方法制备的半抗原 - SRBC非常稳定，与将这些半抗原与红细胞偶联的其他方法相比具有显著优势。
