发光免疫测定法（LIA）：一种通过化学发光监测的固相免疫测定法。
Luminescence immunoassay (LIA): a solid-phase immunoassay monitored by chemiluminescence.
作者信息
Olsson T, Brunius G, Carlsson H E, Thore A
出版信息
J Immunol Methods. 1979;25(2):127-35. doi: 10.1016/0022-1759(79)90048-6.
A luminescence immunoassay (LIA) has been developed utilizing the chemiluminescent luminol reaction with heme as catalyst. Rabbit antibody against human serum albumin was quantitated in antigen coated plastic tubes using commercially available goat anti-rabbit IgG conjugated to horseradish peroxidase which was the source of heme. The measurable range of antibody is considerably wider by LIA than by enzyme immunoassays. The time to develop and measure activity is short and constant which makes LIA suitable for automation. In its present form, LIA is slightly less sensitive but has better day-to-day reproducibility than corresponding enzymes immunoassays.
利用以血红素为催化剂的化学发光鲁米诺反应开发了一种发光免疫测定法（LIA）。在包被抗原的塑料管中，使用与辣根过氧化物酶偶联的市售山羊抗兔IgG（其为血红素来源）对兔抗人血清白蛋白抗体进行定量。与酶免疫测定法相比，LIA可检测的抗体范围要宽得多。显色和测量活性的时间短且恒定，这使得LIA适用于自动化。就其目前的形式而言，LIA的灵敏度略低，但与相应的酶免疫测定法相比，具有更好的日常重复性。
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