Department of Food Engineering, Graduate School of Science and Engineering, Hacettepe University, Beytepe Campus, 06800, Ankara, Turkey.
FoodOmics Laboratory, Department of Food Engineering, Hacettepe University, Beytepe Campus, 06800, Ankara, Turkey.
Mol Biol Rep. 2023 Jun;50(6):4813-4822. doi: 10.1007/s11033-023-08408-2. Epub 2023 Apr 8.
Reliable and efficient methods for detecting genetically modified organisms (GMOs) in unprocessed and processed food will be essential for establishing an effective system for traceability all along the supply chain. It is important to understand the detection of GMOs following microwave treatment, which is a common processing method used in various food products such as flours. Therefore, this study aimed to detect the presence of Cauliflower mosaic virus (CaMV) 35S promoter (P-35S), Figwort mosaic virus (FMV) promoter (P-FMV), and T-NOS (nopaline synthase terminator) genetic elements in DNA samples from untreated and microwave-treated genetically modified (GM) cereal flour samples using the qualitative polymerase chain reaction (PCR) based screening method.
DNA was extracted from all samples, and the efficiency of the qualitative PCR screening technique was tested by the verification studies. We performed an inhibition study with plant-specific actin (ACT) gene to the effectiveness of confirming the DNA extraction method. Then, we made the confirming of the qualitative PCR system by method performance testing criteria. The high quality and quantity of the DNA extracts from untreated and microwave-treated flour samples indicated the applicability of qualitative PCR screening assays. The results showed that microwave radiation does not significantly impact the genetic element screening in flour materials.
Untreated and microwave-treated flour samples had amplifiable DNA for the simultaneous screening of three genetic elements. The qualitative screening tests conducted in this study produced dependable outcomes, thus, can be successfully used for monitoring in control laboratories.
可靠且高效的方法可用于检测未经加工和加工食品中的转基因生物（GMO），这对于建立整个供应链可追溯性的有效系统至关重要。了解微波处理后 GMO 的检测非常重要，微波处理是各种食品（如面粉）中常用的加工方法。因此，本研究旨在使用基于定性聚合酶链反应（PCR）的筛选方法，检测未经处理和微波处理的转基因谷物面粉样品的 DNA 样本中是否存在花椰菜花叶病毒（CaMV）35S 启动子（P-35S）、瓜叶病毒（FMV）启动子（P-FMV）和 T-NOS（胭脂碱合成酶终止子）遗传元件。
从所有样品中提取 DNA，并通过验证研究测试定性 PCR 筛选技术的效率。我们使用植物特异性肌动蛋白（ACT）基因进行抑制研究，以验证 DNA 提取方法的有效性。然后，我们通过方法性能测试标准来确认定性 PCR 系统。未经处理和微波处理的面粉样品中高质量和数量的 DNA 提取物表明定性 PCR 筛选分析的适用性。结果表明，微波辐射不会对面粉材料中的遗传元件筛选产生重大影响。
未经处理和微波处理的面粉样品具有可用于同时筛选三种遗传元件的扩增 DNA。本研究中进行的定性筛选测试产生了可靠的结果，因此可成功用于控制实验室的监测。
