School of Medicine, Department of Anatomy, Pamukkale University, 20070, Denizli, Turkey.
School of Medicine, Department of Physiology, Pamukkale University, 20070, Denizli, Turkey.
J Bone Miner Metab. 2023 Jul;41(4):443-456. doi: 10.1007/s00774-023-01416-z. Epub 2023 Apr 8.
Osteoporosis is a major health problem that is very common worldwide and is characterized by both low bone density and deterioration in bone quality. New treatment options without side effects have become an active area of research in recent years. This study was designed to investigate the preventive effects of resveratrol on bone quality deterioration caused by ovariectomy.
Sixty rats were randomly divided into five groups (12 animals per group): Control, Sham-operated (SHAM), ovariectomized (OVX), OVX + Resveratrol-40 mg/kg/day (OVX + Res40), OVX + Resveratrol-80 mg/kg/day (OVX + Res80). Resveratrol was administered by oral gavage (40 and 80 mg/kg/day) for ten weeks. Micro-CT measurements, biomechanical testing, Raman spectroscopy analysis, and RT-PCR analysis were performed. ALP, OCN, TAS, and TOS levels were also measured from blood serum.
Bone strength, bone volume/total volume, trabecular volume, and trabecular thickness were higher in the OVX + RES-80 group than in the OVX group. Resveratrol increased osteogenic differentiation, as the expression of osteogenic markers ALP, Col1A1, Runx2, OPG, OCN increased in both OVX + RES-80 and OVX + RES-40 groups compared to the OVX group. 80 mg/kg/day resveratrol administration decreased the levels of ALP, OCN and TOS in ovariectomized rats. Raman spectroscopy findings showed a preventive effect of resveratrol administration against ovariectomy-induced deterioration in biophysiochemical properties of bone tissue.
This study revealed that administration of different doses of 80 mg/kg/day and 40 mg/kg/day of resveratrol had protective effects on bone quality deterioration caused by ovariectomy.
骨质疏松症是一种全球性的重大健康问题，其特征是骨密度低和骨质量恶化。近年来，没有副作用的新治疗选择成为研究的活跃领域。本研究旨在探讨白藜芦醇对去卵巢引起的骨质量恶化的预防作用。
将 60 只大鼠随机分为五组（每组 12 只动物）：对照组、假手术组（SHAM）、去卵巢组（OVX）、去卵巢+白藜芦醇 40mg/kg/天组（OVX+Res40）、去卵巢+白藜芦醇 80mg/kg/天组（OVX+Res80）。白藜芦醇通过口服灌胃（40 和 80mg/kg/天）给药 10 周。进行 micro-CT 测量、生物力学测试、拉曼光谱分析和 RT-PCR 分析。还从血清中测量碱性磷酸酶（ALP）、骨钙素（OCN）、总抗氧化能力（TAS）和总氧化应激（TOS）水平。
与 OVX 组相比，OVX+RES-80 组的骨强度、骨体积/总体积、小梁体积和小梁厚度更高。白藜芦醇增加了成骨分化，因为与 OVX 组相比，OVX+RES-80 和 OVX+RES-40 组的成骨标志物碱性磷酸酶（ALP）、Col1A1、Runx2、OPG、OCN 的表达增加。80mg/kg/天白藜芦醇给药降低了去卵巢大鼠的 ALP、OCN 和 TOS 水平。拉曼光谱结果表明，白藜芦醇给药对去卵巢引起的骨组织生物物理化学性质恶化具有预防作用。
本研究表明，给予 80mg/kg/天和 40mg/kg/天的不同剂量白藜芦醇对去卵巢引起的骨质量恶化具有保护作用。
