Farzaneh F, Pearson C K
J Embryol Exp Morphol. 1978 Dec;48:101-8.
A method for isolating nuclei from Xenopus laevis embryos has been developed. This procedure enables the isolation of nuclei, free from contamination with yolk and pigment granules, at all stages of embryoic development. Using this method the nuclear yield is 60--70% of the estimated number of cells in the embryo. The DNA, RNA, histone and non-histone protein content of these nuclei during embryogenesis (from early cleavage to the swimming tadpole stage) has been measured.
已开发出一种从非洲爪蟾胚胎中分离细胞核的方法。该程序能够在胚胎发育的所有阶段分离出不含卵黄和色素颗粒污染的细胞核。使用这种方法，细胞核产量为胚胎中估计细胞数的60%-70%。已测量了这些细胞核在胚胎发生过程中（从早期卵裂到蝌蚪游动阶段）的DNA、RNA、组蛋白和非组蛋白含量。
