大鼠肾上腺嗜铬细胞在体外培养时对α- latrotoxin变得敏感：神经生长因子的作用。

Rat adrenal chromaffin cells become sensitive to alpha-latrotoxin when cultured in vitro: the effect of nerve growth factor.

作者信息

Kobayashi H, Izumi F, Meldolesi J

出版信息

Neurosci Lett. 1986 Mar 28;65(1):114-8. doi: 10.1016/0304-3940(86)90130-8.

Abstract

Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, alpha-latrotoxin (alpha-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to alpha-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to alpha-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

摘要

先前的研究表明，在完整的大鼠体内，嗜铬细胞对突触前刺激毒素α- 拉曲毒素（α-LTx）不敏感。相比之下，大鼠肾上腺嗜铬细胞的原代培养物会通过释放储存的儿茶酚胺对α-LTx作出反应。毒素诱导的反应在来自幼年（8 - 10日龄）动物的培养物中比成年（40 - 50日龄）动物的培养物中更大，并且当培养时间从4天延长到20天时反应会增加。在存在神经生长因子的情况下培养的细胞中观察到了更大的反应，神经生长因子这种处理会诱导出类似神经元的表型。对α-LTx敏感性的获得似乎是嗜铬细胞向神经元分化的早期标志。