Cimrin Tulay, Alasahan Sema, Kazak Filiz, Kutlu Tuncer, Kisacam Mehmet Ali
Department of Animal Science, Faculty of Agriculture, University of Hatay Mustafa Kemal, Hatay, Türkiye.
Department of Animal Breeding, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, Hatay, Türkiye.
Vet Res Forum. 2023;14(3):121-129. doi: 10.30466/vrf.2022.549517.3404. Epub 2023 Mar 15.
This research aimed to evaluate the effect of different doses of black cumin ( L.) seed (BCS) on growth performance, blood parameters, liver oxidant/anti-oxidant levels and fatty liver syndrome in quails. Four hundred and thirty-two unsexed (male and female) three-day-old Japanese quail () chicks were divided into four treatment groups (108 chicks per group) with six replicates (18 chicks per replicate). Control and experimental groups were fed for 35 days with basal quail feed including 0.00, 0.50, 1.00 and 2.00% BCS supplement, respectively. At the end, a total of 96 quails, 24 from each group (12 females and 12 males) were slaughtered. The BCS-addition did not affect the growth performance in the experimental groups compared to the control group. Addition of BCS to the diet significantly decreased serum aspartate aminotransferase, lactate dehydrogenase and urea amounts compared to the controls. Whereas, cholesterol decreased significantly with the addition of only 1.00% and low-density lipoprotein with the addition of 0.50 and 1.00% BCS compared to the controls. Liver glutathione levels significantly elevated in 0.50 and 1.00% BCS fed groups; while, glutathione peroxidase levels significantly decreased in 1.00 and 2.00% BCS fed groups. Adding 1.00 and 2.00% BCS to the feed reduced fatty liver incidence in male quails. It is concluded that adding 0.50 and 1.00% BCS positively affects the blood and liver parameters; therefore, BCS may be suggested as an anti-oxidant source to help protect hepatocytes against tissue damage as it has a significant effect on maintaining oxidant and anti-oxidant balance.
本研究旨在评估不同剂量的黑种草（Nigella sativa L.）籽（BCS）对鹌鹑生长性能、血液参数、肝脏氧化/抗氧化水平及脂肪肝综合征的影响。432只三日龄未分性别的日本鹌鹑（Coturnix japonica）雏鸡被分为四个处理组（每组108只雏鸡），每组六个重复（每个重复18只雏鸡）。对照组和实验组分别用添加了0.00%、0.50%、1.00%和2.00% BCS的基础鹌鹑饲料喂养35天。最后，每组宰杀24只鹌鹑（12只雌性和12只雄性），共96只。与对照组相比，添加BCS对实验组的生长性能没有影响。与对照组相比，在日粮中添加BCS显著降低了血清天冬氨酸转氨酶、乳酸脱氢酶和尿素含量。然而，与对照组相比，仅添加1.00% BCS时胆固醇显著降低，添加0.50%和1.00% BCS时低密度脂蛋白显著降低。在饲喂0.50%和1.00% BCS的组中，肝脏谷胱甘肽水平显著升高；而在饲喂1.00%和2.00% BCS的组中，谷胱甘肽过氧化物酶水平显著降低。在饲料中添加1.00%和2.00% BCS可降低雄性鹌鹑的脂肪肝发病率。研究得出结论，添加0.50%和1.00% BCS对血液和肝脏参数有积极影响；因此，BCS可作为一种抗氧化剂来源，有助于保护肝细胞免受组织损伤，并对维持氧化和抗氧化平衡有显著作用。
