Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Canada.
Eur J Gastroenterol Hepatol. 2010 Jul;22(7):826-34. doi: 10.1097/MEG.0b013e328336000d.
BACKGROUND/AIMS: This study examines the modifying effects of thymoquinone (TQ), an agent with antioxidant and hypolipidemic properties, on reactive oxygen species, antioxidant activity, and steatosis in livers of hyperlipidemic rabbit.
Oxidative stress was induced in New Zealand White rabbit by a high-cholesterol diet for 8 weeks. Four groups of six animals each were divided into a control group; a high cholesterol group (received 1% cholesterol diet); a high cholesterol/low TQ group (received 1% cholesterol diet plus TQ 10 mg/kg/day), and a high cholesterol/high TQ (20 mg/kg/day) group. Blood samples were taken at the end of the study and examined for fasting serum glucose, insulin, and aminotransaminases. Hepatic tissue samples were examined for malondialdehyde, protein carbonyls, antioxidant enzymes, and steatosis.
There was severe hepatic steatosis and elevated serum alanine aminotransferase in the high cholesterol group (group II) but not in the high cholesterol and low or high TQ groups (groups III, IV). The hepatic reactive oxygen species activity in the high cholesterol group was significantly higher while the antioxidant enzymes were lower (P<0.05) when compared with the control (group I) or either of the high cholesterol and TQ groups.
TQ attenuates hepatic oxidative stress in fatty liver injury induced by high-cholesterol diet in rabbits.
背景/目的：本研究旨在探讨具有抗氧化和降血脂作用的化合物——胸腺醌（TQ）对高脂血症兔肝脏活性氧、抗氧化活性和脂肪变性的修饰作用。
通过高脂饮食 8 周诱导新西兰白兔产生氧化应激。将 6 只动物分为 4 组，每组 6 只，分别为对照组；高胆固醇组（给予 1%胆固醇饮食）；高胆固醇/低 TQ 组（给予 1%胆固醇饮食加 TQ 10mg/kg/天）和高胆固醇/高 TQ 组（20mg/kg/天）。在研究结束时采集血样，检测空腹血糖、胰岛素和氨基转移酶。检查肝组织样本中的丙二醛、蛋白质羰基、抗氧化酶和脂肪变性。
高胆固醇组（第 II 组）出现严重的肝脂肪变性和血清丙氨酸氨基转移酶升高，但高胆固醇和低或高 TQ 组（第 III、IV 组）没有。与对照组（第 I 组）或高胆固醇和 TQ 组相比，高胆固醇组的肝活性氧物质活性显著升高，而抗氧化酶活性降低（P<0.05）。
TQ 可减轻由高胆固醇饮食引起的兔脂肪肝损伤中的肝氧化应激。
