Zhang Hua, Qin Yufen, Jia Mengting, Li Lei, Zhang Wei, Li Lu, Zhang Zhixin, Liu Yanrong
Department of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University Jining 272029, Shandong, China.
School of Clinical Medicine, Jining Medical University Jining 272029, Shandong, China.
Am J Cancer Res. 2023 Mar 15;13(3):964-975. eCollection 2023.
Patients with gastric cancer exhibit considerable genetic and phenotypic heterogeneity, necessitating the establishment of a model for personalized medicine screening. Here, we successfully established three-dimensional cell spheroids (3D cell spheroids) from fresh gastric cancer tissues (n = 30) as in vitro models for further therapeutic screening. Hematoxylin-eosin and immunohistochemical staining of whole spheroids and parental tumor tissues revealed that the 3D cell spheroids recapitulated the parental tissue structure and maintained the histological characteristics of the parental tumor tissue during long-term expansion in vitro. Further, transcriptome sequencing verified that the cell spheroids could recapitulate the gene expression profile characteristics of the parental tumor tissue. Drug susceptibility testing of the 3D cell spheroids demonstrated that these cell spheroids can be used as a reliable model for drug prediction.
胃癌患者表现出显著的遗传和表型异质性，因此有必要建立一种个性化医学筛查模型。在此，我们成功地从30例新鲜胃癌组织中构建了三维细胞球体（3D细胞球体）作为体外模型，用于进一步的治疗性筛查。对整个球体和亲本肿瘤组织进行苏木精-伊红染色和免疫组化染色显示，3D细胞球体在体外长期扩增过程中重现了亲本组织结构，并保留了亲本肿瘤组织的组织学特征。此外，转录组测序证实细胞球体能够重现亲本肿瘤组织的基因表达谱特征。3D细胞球体的药敏试验表明，这些细胞球体可作为药物预测的可靠模型。
