Cai Pingping, Casas Christian J, Plancarte Gabriel Quintero, Hua Lisa L, Mikawa Takashi
bioRxiv. 2024 Mar 10:2023.03.27.534352. doi: 10.1101/2023.03.27.534352.
Little is known about how distance between homologous chromosomes are controlled during the cell cycle. Here, we show that the distribution of centromere components display two discrete clusters placed to either side of the centrosome and apical/basal axis from prophase to G interphase. 4-Dimensional live cell imaging analysis of centromere and centrosome tracking reveals that centromeres oscillate largely within one cluster, but do not cross over to the other cluster. We propose a model of an axis-dependent ipsilateral restriction of chromosome oscillations throughout mitosis.
关于在细胞周期中同源染色体之间的距离是如何控制的，目前所知甚少。在这里，我们表明，从前期到G1期，着丝粒组件的分布呈现出位于中心体和顶/基轴两侧的两个离散簇。对着丝粒和中心体追踪的四维活细胞成像分析表明，着丝粒主要在一个簇内振荡，但不会跨越到另一个簇。我们提出了一个在整个有丝分裂过程中染色体振荡的轴依赖性同侧限制模型。
