Kim Janghyun, Lee Bora, Kim Young, Kim Byeong C, Kim Joon-Tae, Cho Hyong-Ho
Department of Neurology, Chonnam National University Hospital, Gwangju 61469, Korea.
Department of Biochemistry, Chonnam National University Medical School, Gwangju 61469, Korea.
Genomics Inform. 2023 Mar;21(1):e2. doi: 10.5808/gi.22061. Epub 2023 Mar 31.
Microglia, similar to peripheral macrophages, are the primary immune cells of the central nervous system (CNS). Microglia exist in the resting state in the healthy CNS, but can be activated and polarized into either M1 or M2 subtypes for immune defense and the maintenance of CNS homeostasis by multiple stimuli. Several long noncoding RNAs (lncRNAs) mediate human inflammatory diseases and neuropathologies by regulating their target genes. However, the function of common lncRNAs that contribute to microglial activation remains unclear. Thus, we used bioinformatic approaches to identify common lncRNAs involved in microglial activation in vitro. Our study identified several lncRNAs as common regulators of microglial activation. We identified 283 common mRNAs and 53 common lncRNAs during mouse M1 microglial activation processes, whereas 26 common mRNAs and five common lncRNAs were identified during mouse M2 microglial activation processes. A total of 648 common mRNAs and 274 common lncRNAs were identified during the activation of human M1 microglia. In addition, we identified 1,920 common co-expressed pairs in mouse M1 activation processes and 25 common co-expressed pairs in mouse M2 activation processes. Our study provides a comprehensive understanding of common lncRNA expression profiles in microglial activation processes in vitro. The list of common lncRNAs identified in this study provides novel evidence and clues regarding the molecular mechanisms underlying microglial activation.
小胶质细胞与外周巨噬细胞类似，是中枢神经系统（CNS）的主要免疫细胞。在健康的中枢神经系统中，小胶质细胞处于静息状态，但可被多种刺激激活并极化为M1或M2亚型，以进行免疫防御并维持中枢神经系统的稳态。几种长链非编码RNA（lncRNA）通过调节其靶基因介导人类炎症性疾病和神经病理学。然而，促成小胶质细胞激活的常见lncRNA的功能仍不清楚。因此，我们使用生物信息学方法来鉴定体外参与小胶质细胞激活的常见lncRNA。我们的研究鉴定了几种lncRNA作为小胶质细胞激活的常见调节因子。在小鼠M1小胶质细胞激活过程中，我们鉴定出283个常见的mRNA和53个常见的lncRNA，而在小鼠M2小胶质细胞激活过程中，鉴定出26个常见的mRNA和5个常见的lncRNA。在人类M1小胶质细胞激活过程中，共鉴定出648个常见的mRNA和274个常见的lncRNA。此外，我们在小鼠M1激活过程中鉴定出1920个常见的共表达对，在小鼠M2激活过程中鉴定出25个常见的共表达对。我们的研究全面了解了体外小胶质细胞激活过程中常见lncRNA的表达谱。本研究中鉴定出的常见lncRNA列表为小胶质细胞激活的分子机制提供了新的证据和线索。
