Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, Sichuan, China.
College of Life Science, Sichuan Normal University, Chengdu 610066, Sichuan, China.
J Appl Microbiol. 2023 Apr 3;134(4). doi: 10.1093/jambio/lxad069.
To investigate the capability, properties, and molecular mechanism of inulin fermentation by lactic acid bacteria (LAB) from Sichuan pickle.
A total of 79 LAB strains were purified from 30 aged Sichuan pickle brine samples, and only 21 Lactiplantibacillus plantarum strains (26.58%, 21/79) derived from 15 samples grew well through utilizing inulin as a carbon source. The fermentation tests through using long-chain inulin (lc-inulin) as a carbon source showed that only 6 L. plantarum strains grew well, while other 15 strains could only utilize short-chain oligofructose (FOS), and thin-layer chromatography analysis evidenced a strain specificity of inulin consumption patterns. Lactiplantibacillus plantarum YT041 is a vigorous inulin fermenter, and whole genome sequencing data revealed that sacPTS1 and fosRABCDXE operons might be associated with the fermentation of FOS and lc-inulin, respectively.
The phenotype of inulin consumption is commonly present in LAB from Sichuan pickle, which is strain-specific and largely depends on their specific ecological niche and degree of polymerization.
研究来源于四川泡菜的乳酸菌（LAB）发酵菊粉的能力、特性和分子机制。
从 30 个老化的四川泡菜卤水中纯化出 79 株 LAB 菌株，只有 15 个样本中的 21 株植物乳杆菌（26.58%，21/79）能够很好地利用菊粉作为碳源生长。通过使用长链菊粉（lc-inulin）作为碳源的发酵试验表明，只有 6 株植物乳杆菌菌株生长良好，而其他 15 株菌株只能利用短链低聚果糖（FOS），薄层色谱分析证明了菊粉消耗模式具有菌株特异性。植物乳杆菌 YT041 是一种强劲的菊粉发酵剂，全基因组测序数据表明 sacPTS1 和 fosRABCDXE 操纵子可能分别与 FOS 和 lc-inulin 的发酵有关。
来源于四川泡菜的 LAB 普遍存在菊粉利用表型，这种表型具有菌株特异性，很大程度上取决于其特定的生态位和聚合度。
