Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Lavale, Pune, 412115, India.
Molecular and Cellular Biology Program, Dartmouth College, Hanover, NH, 03755, USA.
Sci Rep. 2022 Feb 4;12(1):1940. doi: 10.1038/s41598-022-05850-3.
Lactiplantibacillus plantarum is one of the most diverse species of lactic acid bacteria found in various habitats. The aim of this work was to perform preliminary phenotypic and genomic characterization of two novel and potentially probiotic L. plantarum strains isolated from Indian foods, viz., dhokla batter and jaggery. Both the strains were bile and acid tolerant, utilized various sugars, adhered to intestinal epithelial cells, produced exopolysaccharides and folate, were susceptible for tetracycline, erythromycin, and chloramphenicol, did not cause hemolysis, and exhibited antimicrobial and plant phenolics metabolizing activities. The genetic determinants of bile tolerance, cell-adhesion, bacteriocins production, riboflavin and folate biosynthesis, plant polyphenols utilization, and exopolysaccharide production were found in both the strains. One of the strains contained a large number of unique genes while the other had a simultaneous presence of glucansucrase and fructansucrase genes which is a rare trait in L. plantarum. Comparative genome analysis of 149 L. plantarum strains highlighted high variation in the cell-adhesion and sugar metabolism genes while the genomic regions for some other properties were relatively conserved. This work highlights the unique properties of our strains along with the probiotic and technically important genomic features of a large number of L. plantarum strains.
植物乳杆菌是存在于各种生境中的最具多样性的乳酸杆菌之一。本工作旨在对从印度食品（即 dhokla 面糊和糖蜜）中分离得到的两株新型潜在益生菌植物乳杆菌进行初步表型和基因组特征分析。两株菌均具有耐胆汁和耐酸特性，能够利用多种糖，黏附于肠上皮细胞，产生胞外多糖和叶酸，对四环素、红霉素和氯霉素敏感，不引起溶血，并表现出抗菌和植物酚类代谢活性。两株菌均存在胆汁耐受、细胞黏附、细菌素产生、核黄素和叶酸生物合成、植物多酚利用和胞外多糖产生的遗传决定因子。其中一株菌含有大量独特的基因，而另一株菌同时存在葡聚糖酶和果糖苷酶基因，这在植物乳杆菌中是罕见的特征。对 149 株植物乳杆菌的比较基因组分析突出了细胞黏附性和糖代谢基因的高度变异性，而其他一些特性的基因组区域则相对保守。本工作突出了我们菌株的独特特性，以及大量植物乳杆菌菌株的益生菌和技术上重要的基因组特征。
