Federal Institute of Education, Science and Technology of Bahia, Campus Xique-Xique, Xique-Xique, Bahia, Brazil.
Brazilian Agricultural Research Corporation (Embrapa Cerrados), Brasília, Federal District, Brazil.
Methods Mol Biol. 2023;2647:105-119. doi: 10.1007/978-1-0716-3064-8_5.
Mammals are routinely cloned by introducing somatic nuclei into enucleated oocytes. Cloning contributes to propagating desired animals, to germplasm conservation efforts, among other applications. A challenge to more broader use of this technology is the relatively low cloning efficiency, which inversely correlates with donor cell differentiation status. Emerging evidence suggests that adult multipotent stem cells improve cloning efficiency, while the greater potential of embryonic stem cells for cloning remains restricted to the mouse. The derivation of pluripotent or totipotent stem cells from livestock and wild species and their association with modulators of epigenetic marks in donor cells should increase cloning efficiency.
哺乳动物通常通过将体细胞核引入去核卵母细胞来克隆。克隆有助于繁殖所需的动物，进行种质保存等应用。该技术的广泛应用面临一个挑战，即克隆效率相对较低，这与供体细胞的分化状态呈负相关。新出现的证据表明，成年多能干细胞可提高克隆效率，而胚胎干细胞在克隆方面具有更大的潜力，但仍仅限于小鼠。从家畜和野生动物中获得多能或全能干细胞，并将其与供体细胞中的表观遗传标记调节剂相关联，应该会提高克隆效率。
