Department of Animal Science, University of California, Davis, CA, USA.
Instituto de Investigaciones Biotecnológicas, CONICET, Universidad Nacional de San Martín, Buenos Aires, Argentina.
Methods Mol Biol. 2023;2647:305-315. doi: 10.1007/978-1-0716-3064-8_17.
Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem cell lines to valuable and well-characterized animals. In this chapter, we describe a step-by-step procedure for deriving bovine embryonic stem cells from whole blastocysts produced by somatic cell nuclear transfer. This simple method requires minimal manipulation of blastocyst-stage embryos, relies on commercially available reagents, supports trypsin passaging, and allows the generation of stable primed pluripotent stem cell lines in 3-4 weeks.
从体细胞核移植胚胎中衍生牛胚胎干细胞使我们能够获得与有价值且特征良好的动物遗传匹配的多能干细胞系。在本章中，我们描述了一种从体细胞核移植产生的整个囊胚中衍生牛胚胎干细胞的逐步程序。该简单方法对囊胚期胚胎的操作要求最少，依赖于商业上可获得的试剂，支持胰蛋白酶传代培养，并允许在 3-4 周内产生稳定的初始多能干细胞系。
