罗莫辛努阿诺牛品种全血中热休克蛋白基因的表达谱分析。
Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed.
作者信息
Taborda-Charris Juan Camilo, Rodríguez-Hernández Roy, Herrera-Sánchez María Paula, Uribe-García Heinner Fabian, Otero-Arroyo Rafael J, Naranjo-Gomez Juan Sebastian, Lozano-Villegas Kelly Johanna, Rondón-Barragán Iang Schroniltgen
机构信息
Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.
Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.
出版信息
Vet World. 2023 Mar;16(3):601-606. doi: 10.14202/vetworld.2023.601-606. Epub 2023 Mar 24.
BACKGROUND AND AIM
Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed.
MATERIALS AND METHODS
Real-time polymerase chain reaction was performed to analyze the transcript of , , , and in the whole blood of Romosinuano under different temperature-humidity indices (THIs).
RESULTS
The expression levels of the and genes at the high-THI level were higher (p = 0.0011 and p = 0.0003, respectively) than those at the low-THI level. In addition, no differences in the expression levels of the and genes were detected between the two THIs.
CONCLUSION
The overexpression of and genes play an important role in protecting cells from damage induced by heat stress.
背景与目的
热休克蛋白是高度保守的蛋白质，作为分子伴侣在热应激反应中表达。本研究旨在确定从罗莫辛努阿诺克里奥尔品种获得的全血中与热应激反应相关基因的表达谱。
材料与方法
采用实时聚合酶链反应分析不同温湿度指数（THI）下罗莫辛努阿诺全血中 、 、 和 的转录本。
结果
高THI水平下 基因和 基因的表达水平分别高于低THI水平（分别为p = 0.0011和p = 0.0003）。此外，在两种THI之间未检测到 基因和 基因表达水平的差异。
结论
和 基因的过表达在保护细胞免受热应激诱导的损伤中起重要作用。
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