Ardani Laily Rinda, Marlida Yetti, Zain Mardiati, Jamsari Jamsari, Fassah Dilla Mareistia
PMDSU Program, Graduate Program, Faculty of Animal Science, Andalas University, Padang, West Sumatera, Indonesia.
Department of Animal Nutrition, Faculty of Animal Science Andalas University, Padang, West Sumatera, Indonesia.
Vet World. 2023 Feb;16(2):395-402. doi: 10.14202/vetworld.2023.395-402. Epub 2023 Feb 28.
Probiotic supplementation can assist with manipulating the rumen microbial ecosystem. Lactic acid bacteria and yeast from fermented fish (Budu) as the indigenous food from West Sumatra, Indonesia, are potential probiotics for livestock. This study aims to select the best candidate lactic acid bacteria and yeast strains from fermented fish as ruminant probiotics and evaluate the effect of their supplementation on the characteristics of rumen fermentation, feed digestion, and total gas production .
This study used nine treatments, performed in triplicate, in a completely randomized design. The substrate ratio comprised of 70% forage and 30% concentrate. Five lactic acid bacteria and three yeast isolates were used in this study. Treatments were as follows: T0: control (basal diet); T1: T0 + strain 3347; T2: T0 + strain 5296; T3: T0 + JCM 16178; T4: T0 + strain LH991; T5: T0 + strain 6902; T6: T0 + strain B-5P; T7: T0 + strain CBS 5147; and T8: T0 + commercial yeast (). The lactic acid bacteria inoculum contained 1.02 × 10 colony-forming unit (CFU)/mL, while the yeast inoculum contained 1.5 × 10 CFU/mL.
The results showed that four lactic acid bacteria and three yeast produced a higher total gas yield (104-183.33 mL) compared to the control (103 mL). Supplementation with lactic acid bacteria in the rumen fermentation showed dry matter digestibility of 63%-70% and organic matter digestibility (OMD) of 64%-71%. We observed that total volatile fatty acid (VFA) production in all treatments was significantly higher (86-121 mM) compared to the control (81 mM). The concentration of NH production was higher in all treatments (12.33-16.83 mM) than in the control (12.25 mM). Meanwhile, the probiotic supplementation did not cause a significant change in the rumen pH (6.86-7.12). Supplementation with the lactic acid bacteria strain LH991 consistently demonstrated the best results from the parameters of dry and OMD (70.29% and 71.16%, respectively), total VFA (121.67 mM), NH (16.83 mM), and total gas production (149.17 mL). The best results were observed from the yeast candidate strain B-5P, where the results were dry and OMD (67.64% and 69.55% respectively), total VFA (96.67 mM), NH (13.42 mM), and total gas production (183.33 mL).
Based on the obtained results, lactic acid bacteria strain LH991 and yeast strain B-5P are attractive candidates to be utilized as probiotics for ruminants based on their potential to improve rumen fermentation . This probiotic supplementation can increase the digestibility of feed ingredients, production of total VFA and NH, and total gas produced.
补充益生菌有助于调控瘤胃微生物生态系统。来自印度尼西亚西苏门答腊本土食物发酵鱼（布杜）中的乳酸菌和酵母是家畜潜在的益生菌。本研究旨在从发酵鱼中筛选出最佳的乳酸菌和酵母菌株作为反刍动物益生菌，并评估其添加对瘤胃发酵特性、饲料消化率和总产气量的影响。
本研究采用完全随机设计，设置9个处理组，每组3个重复。底物比例为70%粗饲料和30%精饲料。本研究使用了5株乳酸菌和3株酵母分离株。处理组如下：T0：对照组（基础日粮）；T1：T0 + 3347菌株；T2：T0 + 5296菌株；T3：T0 + JCM 16178；T4：T0 + LH991菌株；T5：T0 + 6902菌株；T6：T0 + B - 5P菌株；T7：T0 + CBS 5147菌株；T8：T0 + 商业酵母（）。乳酸菌接种物浓度为1.02×10菌落形成单位（CFU）/mL，酵母接种物浓度为1.5×10 CFU/mL。
结果表明，与对照组（103 mL）相比，4株乳酸菌和3株酵母产生的总产气量更高（104 - 183.33 mL）。瘤胃发酵中添加乳酸菌后，干物质消化率为63% - 70%，有机物消化率（OMD）为64% - 71%。我们观察到，所有处理组的总挥发性脂肪酸（VFA）产量（86 - 121 mM）均显著高于对照组（81 mM）。所有处理组的NH产量浓度（12.33 - 16.83 mM）均高于对照组（12.25 mM）。同时，补充益生菌对瘤胃pH值（6.86 - 7.12）无显著影响。添加乳酸菌LH991菌株在干物质和OMD参数（分别为70.29%和71.16%）、总VFA（121.67 mM）、NH（16.83 mM）和总产气量（149.17 mL）方面始终表现出最佳效果。酵母候选菌株B - 5P的效果最佳，其干物质和OMD结果分别为67.64%和69.55%，总VFA（96.67 mM），NH（13.42 mM），总产气量（183.33 mL）。
基于所得结果，乳酸菌LH991菌株和酵母B - 5P菌株因其改善瘤胃发酵的潜力，是作为反刍动物益生菌的有吸引力的候选菌株。这种益生菌补充可以提高饲料成分的消化率、总VFA和NH的产量以及总产气量。
