Zaki Abdel-Kader A, Aldahmashi Fahad S, Madboli Abd El-Nasser A, Attia Kamal A, Almulhim Fahad S, Albarrak Saleh M
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.
Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Vet World. 2023 Feb;16(2):296-308. doi: 10.14202/vetworld.2023.296-308. Epub 2023 Feb 15.
Anti-sperm antibodies (ASAs) treatment continued to be neglected. This study aimed to generate ASAs using the testicular sperm aspiration (TSA) rat model, which allowed for investigation of four distinct therapeutic approaches to find potential treatments for ASAs.
Adult Wistar albino male rats were divided into six equal groups (n = 12). The negative control group underwent scrotal sac surgery without having their testicles punctured. Punctures were made in the remaining 5 groups, with one group left untreated to serve as the positive control group. The remaining 4 groups were treated with either dexamethasone (DEX), azathioprine (AZA), frankincense, or anti-ASAs secondary antibodies. For 10 weeks, serum samples were collected every 2 weeks for specific quantification of ASAs. Testis and epididymis tissues were collected for histopathological analysis.
The ASAs concentrations of the positive controls were significantly higher (p ≤ 0.001) than their negative control counterparts during the examined weeks. However, The ASAs indices (%) differed according to the treatment type. While the ASAs indices at the 2 and 4 weeks in the AZA-treated group were significantly reduced compared to the positive control group (p ≤ 0.001), no significant differences were observed at any of the sample collection week for the DEX-treated rats. The ASAs indices were significantly decreased only at weeks 6 and 8 of treatment in the frankincense-treated group (p ≤ 0.001). In the secondary antibodies-treated group, the antibody indices were significantly decreased in all weeks except for samples collected at week 4 (p ≤ 0.001). The testosterone levels reverted to normal only in TSA rats treated with either Frankincense or secondary antibodies, as they were significantly higher than the positive controls (p ≤ 0.05). Tissue samples from the secondary antibody-treated rats showed a generally normal histological appearance.
This study tried to offer realistic therapy suggestions; however, caution should be applied when extrapolating findings from experimental models to meet clinical requirements.
抗精子抗体（ASAs）的治疗一直未得到重视。本研究旨在利用睾丸精子抽吸术（TSA）大鼠模型产生ASAs，以便研究四种不同的治疗方法，寻找ASAs的潜在治疗方案。
将成年Wistar白化雄性大鼠分为六个相等的组（n = 12）。阴性对照组接受阴囊手术，但不穿刺睾丸。其余5组进行穿刺，其中一组不进行治疗作为阳性对照组。其余4组分别用 dexamethasone（DEX）、硫唑嘌呤（AZA）、乳香或抗ASAs二抗进行治疗。在10周内，每2周采集一次血清样本，用于ASAs的特异性定量分析。收集睾丸和附睾组织进行组织病理学分析。
在检查的几周内，阳性对照组的ASAs浓度显著高于阴性对照组（p≤0.001）。然而，ASAs指数（%）因治疗类型而异。与阳性对照组相比，AZA治疗组在第2周和第4周的ASAs指数显著降低（p≤0.001），而DEX治疗的大鼠在任何样本采集周均未观察到显著差异。乳香治疗组仅在治疗的第6周和第8周ASAs指数显著降低（p≤0.001）。在二抗治疗组中，除第4周采集的样本外，所有周的抗体指数均显著降低（p≤0.001）。仅在接受乳香或二抗治疗的TSA大鼠中，睾酮水平恢复正常，因为它们显著高于阳性对照组（p≤0.05）。二抗治疗大鼠的组织样本显示组织学外观总体正常。
本研究试图提供切实可行的治疗建议；然而，将实验模型的研究结果外推以满足临床需求时应谨慎。
