Erciyes University Medical Faculty Department of Medical Biology, Kayseri - Turquia.
Erciyes University Genome and Stem Cell Center, Kayseri - Turquia.
Arq Bras Cardiol. 2023 Apr 7;120(4):e20220169. doi: 10.36660/abc.20220169. eCollection 2023.
Coronary artery disease is a complex disorder that causes death worldwide. One of the genes involved in developing this disease may be PTEN.
This study aimed to investigate the PTEN gene and protein expression in tissue and blood samples taken from coronary bypass surgery patients.
Molecular studies were performed at Erciyes University Genome and Stem Cell Center (GENKOK). Right atrial appendage and blood samples were taken from the central vein of 22 coronary bypass surgery patients before starting and ending cardiopulmonary bypass. PTEN expression was determined using quantitative real-time PCR and western blot analysis. The significance level was accepted as p<0.05.
There was no significant difference in the PTEN gene expression in blood samples taken before and after cardiopulmonary bypass. However, a substantial increase in both protein and gene expression levels of P-PTEN and PTEN was observed in the tissue samples. Myocardial expression of the PTEN gene was significantly increased at the end of the cardiopulmonary bypass. PTEN gene expression in the post-cardiopulmonary bypass period was increased when compared to the pre-bypass period, but it was insignificant when compared to healthy controls.
This study first revealed the role of the PTEN gene by analyzing both mRNA and protein expression in coronary bypass patients, appearing in both myocardial tissue and blood samples. Increased levels of PTEN may be a marker in myocardial tissue for patients with coronary artery disease.
冠状动脉疾病是一种复杂的疾病，在全球范围内导致死亡。参与这种疾病发展的基因之一可能是 PTEN。
本研究旨在调查接受冠状动脉旁路手术患者的组织和血液样本中的 PTEN 基因和蛋白表达。
在埃尔吉耶斯大学基因组和干细胞中心（GENKOK）进行了分子研究。在开始和结束体外循环之前，从 22 名冠状动脉旁路手术患者的右心房附件和中心静脉中采集右心房附件和血液样本。使用定量实时 PCR 和 Western blot 分析来确定 PTEN 表达。置信水平设定为 p<0.05。
体外循环前后采集的血液样本中 PTEN 基因表达无显著差异。然而，在组织样本中观察到 P-PTEN 和 PTEN 的蛋白和基因表达水平均显著增加。体外循环结束时，PTEN 基因在心肌中的表达显著增加。与体外循环前相比，体外循环后 PTEN 基因表达增加，但与健康对照组相比无统计学意义。
本研究首次通过分析冠状动脉旁路患者的 mRNA 和蛋白表达，在心肌组织和血液样本中揭示了 PTEN 基因的作用。PTEN 水平的升高可能是冠状动脉疾病患者心肌组织中的一个标志物。
