Wu Na, Lu Chang, Wang Youjuan, Liu Liuhui, Lu Dingyou, Zhou Ying, He Min, Zhang Xiaobing, Song Guosheng
State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Key Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, China.
Anal Chem. 2023 Apr 25;95(16):6603-6611. doi: 10.1021/acs.analchem.2c05624. Epub 2023 Apr 12.
The total antioxidant capacity (TAC) is a key indicator of the body's resistance to oxidative stress injury in diabetic patients. The measurement of TAC is important for effectively evaluating the redox state to prevent and control the occurrence of diabetes complications. However, there is a lack of a simple, convenient, and reliable method to detect the total antioxidant capacity in diabetes. Herein, we design a novel chemiluminescent platform based on semiconducting polymer nanoparticles-manganese (SPNs-Mn) to detect the total antioxidant capacity of urine in diabetic mice. We synthesize semiconducting polymer nanoparticles with four different structures and discover the ability of Mn to produce singlet oxygen (O) that is employed to excite thiophene-based SPNs (PFODBT) to emit near-infrared chemiluminescence. Notably, the chemiluminescent intensity has a good linear relationship with the concentration of Mn (detection limit: 2.8 μM). Because antioxidants (e.g., glutathione or ascorbic acid) can react with Mn, such a chemiluminescent tool of SPNs (PFODBT)-Mn can detect the glutathione or ascorbic acid with a larger responsive range. Furthermore, the total antioxidant capacity of urine from mice is evaluated via SPNs (PFODBT)-Mn, and there are statistically significant differences between diabetic and healthy mice. Thus, this new chemiluminescent platform of SPNs (PFODBT)-Mn is convenient, efficient, and sensitive, which is promising for monitoring antioxidant therapy of diabetes.
总抗氧化能力（TAC）是糖尿病患者机体抵抗氧化应激损伤的关键指标。TAC的测定对于有效评估氧化还原状态以预防和控制糖尿病并发症的发生具有重要意义。然而，目前缺乏一种简单、便捷且可靠的方法来检测糖尿病患者的总抗氧化能力。在此，我们设计了一种基于半导体聚合物纳米颗粒-锰（SPNs-Mn）的新型化学发光平台，用于检测糖尿病小鼠尿液中的总抗氧化能力。我们合成了四种不同结构的半导体聚合物纳米颗粒，并发现锰产生单线态氧（O）的能力，该单线态氧用于激发基于噻吩的SPNs（PFODBT）发出近红外化学发光。值得注意的是，化学发光强度与锰的浓度具有良好的线性关系（检测限：2.8 μM）。由于抗氧化剂（如谷胱甘肽或抗坏血酸）可以与锰发生反应，因此这种SPNs（PFODBT）-Mn化学发光工具能够在更大的响应范围内检测谷胱甘肽或抗坏血酸。此外，通过SPNs（PFODBT）-Mn评估了小鼠尿液的总抗氧化能力，糖尿病小鼠和健康小鼠之间存在统计学上的显著差异。因此，这种新型的SPNs（PFODBT）-Mn化学发光平台方便、高效且灵敏，有望用于监测糖尿病的抗氧化治疗。
