Sun Xiaoyuan, Huang Yongsheng, Chen Fengming, Gui Guofeng, Li Shengkai
College of Chemistry and Chemical Engineering, Guizhou University of Engineering Science, Bijie, 551700, China.
Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang, 550025, China.
Mikrochim Acta. 2025 Jun 19;192(7):435. doi: 10.1007/s00604-025-07296-6.
A robust oxidase (OXD)-like Pt/C nanozyme is proposed for the estimation of total antioxidant capacity (TAC) in foods. Vulcan XC-72 carbon material serves as both the support and stabilizer of the nanozyme, synthesized using a one-pot solvothermal method. A Pt/C nanozyme-based ascorbic acid (AA) quantitative colorimetric sensing platform was developed. The experimental results showed that the detection range for AA was 3-50 μM, with a detection limit of 0.93 μM, which is comparable with that of related studies. The proposed AA colorimetric could resist the interference from some metal ions, small bioactive molecules, and antioxidants, indicating its superior selectivity. The estimation of TAC in daily foods including commercial beverages and fruit juices was further explored, with results highly consistent with those obtained using the commercial kit detection method. Notably, the preparation process for Pt/C nanozyme was simple and well-suited for large-scale production, and it exhibited superior catalytic activity even after prolonged storage, indicating its substantial potential for real-time TAC detection. This work not only provided a robust strategy for synthesizing highly efficient nanozymes but also developed a convenient method for TAC detection to help individuals make informed dietary choices.
提出了一种用于评估食品中总抗氧化能力（TAC）的类似氧化酶（OXD）的稳健铂/碳纳米酶。Vulcan XC - 72碳材料作为纳米酶的载体和稳定剂，采用一锅溶剂热法合成。开发了基于铂/碳纳米酶的抗坏血酸（AA）定量比色传感平台。实验结果表明，AA的检测范围为3 - 50 μM，检测限为0.93 μM，与相关研究相当。所提出的AA比色法能够抵抗一些金属离子、小生物活性分子和抗氧化剂的干扰，表明其具有优异的选择性。进一步探索了包括商业饮料和果汁在内的日常食品中TAC的评估，结果与使用商业试剂盒检测方法获得的结果高度一致。值得注意的是，铂/碳纳米酶的制备过程简单，适合大规模生产，并且即使在长期储存后仍表现出优异的催化活性，表明其在实时TAC检测方面具有巨大潜力。这项工作不仅为合成高效纳米酶提供了一种稳健的策略，还开发了一种方便的TAC检测方法，以帮助个人做出明智的饮食选择。
