超声诱导细胞破碎及其超微结构表征对蛋白核小球藻增值的研究
Ultrasound-induced cell disintegration and its ultrastructure characterization for the valorisation of Chlorella pyrenoidosa protein.
机构信息
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Innovation Center of Yangtze River Delta, Zhejiang University, Jiashan 314100, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China.
出版信息
Bioresour Technol. 2023 Aug;381:129046. doi: 10.1016/j.biortech.2023.129046. Epub 2023 Apr 10.
Chlorella pyrenoidosa (CP) has great potential for feeding future demands in food, environment, energy, and pharmaceuticals. To achieve this goal, the exploitation of emerging efficient technique such as ultrasound-assisted extraction (UAE) for CP nutrient enrichment is crucial. Here, UAE is deployed for high-efficient CP protein (CPP) valorisation. Compared to conventional solvent extraction (CSE), remarkable mass transfer enhancements with 9-time protein yields and 3-time extraction rate are achieved by ultrasonic cavitation in UAE, indicating UAE can drastically shift intracellular nutrients including proteins and pigments to solvent. Cell morphology and ultrastructure show the different responses of cell wall and membrane, indicating that the cell membrane may play a role in the extraction process, based on which the extremely-low efficiency of CSE and high efficiency of UAE are highlighted. This study provides a solution for future food crisis by extracting CPP and may open a new discussion field in ultrasonic extraction.
蛋白核小球藻（Chlorella pyrenoidosa，CP）在食品、环境、能源和制药等领域具有巨大的应用潜力，可以满足未来的需求。为了实现这一目标，开发新兴的高效技术，如超声辅助提取（ultrasound-assisted extraction，UAE），对于 CP 营养成分的富集至关重要。在这里，我们将 UAE 技术应用于高效 CP 蛋白（CPP）的增值。与传统溶剂提取（conventional solvent extraction，CSE）相比，超声空化在 UAE 中实现了显著的传质增强，CPP 的产率提高了 9 倍，提取速率提高了 3 倍，表明 UAE 可以将包括蛋白质和色素在内的细胞内营养物质剧烈地转移到溶剂中。细胞形态和超微结构显示细胞壁和细胞膜的不同反应，这表明细胞膜可能在提取过程中发挥作用，基于此，我们突出了 CSE 效率极低而 UAE 效率极高的特点。本研究通过提取 CPP 为未来的粮食危机提供了一种解决方案，并可能在超声提取领域开辟一个新的讨论领域。
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