Ding Xunhuan, Fu Yun, Zhou Xuan, Yang Shubing, Cao Yunmeng, Hou Fuxiao, Liu Xiaoli, Sun Tao
College of Life Science, Huaibei Normal University, Huaibei, 235000, China.
Zootaxa. 2022 Nov 28;5213(4):427-440. doi: 10.11646/zootaxa.5213.4.6.
Calliptamus barbarus Costa is a species in genus Calliptamus, belonging to the Calliptaminae, Acrididae, Orthoptera. In the present study, the mitogenome of C. barbarous was determined and annotated to better identify C. barbarous and other related species. The mitogenome was 15,686 bp in length and encoded 37 genes, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The nucleotide composition analysis of the mitogenome showed a strong bias of A/T, with A+T content of 72.7%, being similar to other reported mitogenomes. All the 13-PCGs used typical start codons of ATN and stop codons of TAA/G. All of the transfer RNA genes had typical clover-leaf structure except the missing dihydrouidine (DHU) arm of tRNASer1. Interestingly, we observed two overlapped but conserved regions between ATP8 and ATP6 genes, ND4 and ND4L genes. The results of Ka/Ks implied that CytB is more conservative than COX1. Phylogenetic analyses based on the 13-PCGs from 30 species indicated strong support for the monophyly of Acridoidea and represented the main topology as follows: (Oedipodinae+ (Acridinae+ (Gomphocerinae +((Calliptaminae+Catantopinae) +(Oxyinae+Melanoplinae))))). The phylogenetic relationship indicated that the molecular taxonomy of C. barbarous was consistent with the current morphological classification. The data in the present study will further enrich the mitogenome database of Acridoidea and provided useful clues for further research of C. barbarous on the mitogenome evolution and diversification of Calliptamus.
意大利蝗（Calliptamus barbarus Costa）是蝗属（Calliptamus）的一个物种，隶属于蝗亚科（Calliptaminae）、蝗科（Acrididae）、直翅目（Orthoptera）。在本研究中，测定并注释了意大利蝗的线粒体基因组，以便更好地鉴定意大利蝗及其他相关物种。该线粒体基因组长度为15,686 bp，编码37个基因，包括13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区。线粒体基因组的核苷酸组成分析显示A/T含量有强烈偏向性，A+T含量为72.7%，与其他已报道的线粒体基因组相似。所有13个蛋白质编码基因使用典型的ATN起始密码子和TAA/G终止密码子。除了tRNASer1缺少二氢尿嘧啶（DHU）臂外，所有转运RNA基因都具有典型的三叶草结构。有趣的是，我们观察到ATP8和ATP6基因、ND4和ND4L基因之间存在两个重叠但保守的区域。Ka/Ks结果表明细胞色素b（CytB）比细胞色素氧化酶亚基1（COX1）更保守。基于30个物种的13个蛋白质编码基因的系统发育分析表明，强烈支持蝗总科的单系性，其主要拓扑结构如下：（斑翅蝗亚科+（蝗亚科+（槌角蝗亚科+（（痂蝗亚科+斑腿蝗亚科）+（皱腹蝗亚科+黑蝗亚科）））））。系统发育关系表明，意大利蝗的分子分类与当前的形态学分类一致。本研究中的数据将进一步丰富蝗总科的线粒体基因组数据库，并为进一步研究意大利蝗的线粒体基因组进化和蝗属的多样化提供有用线索。
