Eugin Group, Barcelona, Spain.
Clinica EUGIN, Barcelona, Spain.
Reprod Biomed Online. 2023 Jun;46(6):911-916. doi: 10.1016/j.rbmo.2023.03.003. Epub 2023 Mar 9.
Do morphokinetic parameters vary between male and female preimplantation embryos?
This was a retrospective cohort study of 175 cycles between March 2018 and June 2021 at two reproductive centres. It included time-lapse data from 92 female and 83 male preimplantation embryos exclusively issued from fresh oocyte donation and undergoing intracytoplasmic sperm injection (ICSI). Only fresh elective single-embryo transfers on day 5 were assessed, and the sex of the embryo was confirmed at birth. The morphokinetic parameters analysed were measured in hours post-insemination (hpi). A two-tailed Student's t-test was used to compare the morphokinetics between embryo sexes and a value of P < 0.05 was considered statistically significant.
Following strict inclusion criteria to avoid poor-quality preimplantation embryos, no significant differences were found in morphokinetic parameters when comparing cycles that resulted in female versus male live births for the following: time to pronuclear fading (22.1 ± 2.4 versus 22.4 ± 2.9 hpi; P = 0.52); time to the 2-cell stage (24.6 ± 2.5 versus 25.0 ± 2.5 hpi; P = 0.34); time to the 3-cell stage (35.3 ± 3.3 versus 35.8 ± 3.1 hpi; P = 0.28); time to the 4-cell stage (36.3 ± 3.4 versus 36.9 ± 3.7 hpi; P = 0.20); time to the 5-cell stage (47.9 ± 4.6 versus 48.0 ± 4.8 hpi; P = 0.88); time to the 8-cell stage (54.0 ± 6.5 versus 54.1 ± 6.5 hpi; P = 0.91); time to the start of blastulation (86.3 ± 14.6 versus 85.7 ± 15.5 hpi; P = 0.78); and time to the full blastocyst stage (93.0 ± 16.9 versus 93.2 ± 17.2 hpi; P = 0.94).
There are no significant differences in morphokinetics between male and female preimplantation embryos.
男性和女性胚胎的形态动力学参数是否存在差异？
这是一项回顾性队列研究，纳入了 2018 年 3 月至 2021 年 6 月在两个生殖中心进行的 175 个周期。研究纳入了 92 个女性和 83 个男性胚胎的时间延长数据，这些胚胎均来自新鲜卵母细胞捐赠并进行了胞浆内单精子注射（ICSI）。仅评估了第 5 天新鲜选择性单胚胎移植，胚胎性别在出生时得到确认。分析的形态动力学参数以受精后小时（hpi）测量。使用双尾学生 t 检验比较胚胎性别之间的形态动力学差异，P 值<0.05 被认为具有统计学意义。
在严格纳入标准下，避免了胚胎质量差的情况，在导致女性和男性活产的周期中，以下形态动力学参数无显著差异：原核消失时间（22.1±2.4 与 22.4±2.9 hpi；P=0.52）；2 细胞阶段时间（24.6±2.5 与 25.0±2.5 hpi；P=0.34）；3 细胞阶段时间（35.3±3.3 与 35.8±3.1 hpi；P=0.28）；4 细胞阶段时间（36.3±3.4 与 36.9±3.7 hpi；P=0.20）；5 细胞阶段时间（47.9±4.6 与 48.0±4.8 hpi；P=0.88）；8 细胞阶段时间（54.0±6.5 与 54.1±6.5 hpi；P=0.91）；开始孵化时间（86.3±14.6 与 85.7±15.5 hpi；P=0.78）；以及完全孵化囊胚阶段时间（93.0±16.9 与 93.2±17.2 hpi；P=0.94）。
男性和女性胚胎的形态动力学参数无显著差异。
