INRS-Centre Armand-Frappier Santé Technologie, 531 Boul. des Prairies, Laval, QC, H7V 1B7, Canada.
Institut des Sciences de la Mer, Université du Québec à Rimouski, 310, allée des Ursulines, C.P. 3300, Rimouski, QC, G5L 3A1, Canada.
Sci Rep. 2023 Apr 12;13(1):5971. doi: 10.1038/s41598-023-32690-6.
Establishing long-term microbiome-based monitoring programs is critical for managing and conserving wild fish populations in response to climate change. In most cases, these studies have been conducted on gut and, to a lesser extent, skin (mucus) microbiomes. Here, we exploited the concept of liquid biopsy to study the circulating bacterial microbiome of two Northern halibut species of economic and ecological importance. Amplification and sequencing of the 16S rRNA gene were achieved using a single drop of blood fixed on FTA cards to identify the core blood microbiome of Atlantic and Greenland halibut populations inhabiting the Gulf of St. Lawrence, Canada. We provide evidence that the circulating microbiome DNA (cmDNA) is driven by genetic and environmental factors. More specifically, we found that the circulating microbiome signatures are species-specific and vary according to sex, size, temperature, condition factor, and geographical localization. Overall, our study provides a novel approach for detecting dysbiosis signatures and the risk of disease in wild fish populations for fisheries management, most notably in the context of climate change.
建立长期的基于微生物组的监测计划对于应对气候变化管理和保护野生鱼类种群至关重要。在大多数情况下，这些研究都是在肠道中进行的，在较小程度上也在皮肤（粘液）微生物组中进行。在这里，我们利用液体活检的概念来研究两种具有经济和生态重要性的北方比目鱼物种的循环细菌微生物组。通过在 FTA 卡上固定的一滴血液来扩增和测序 16S rRNA 基因，以鉴定栖息在加拿大圣劳伦斯湾的大西洋和格陵兰比目鱼种群的核心血液微生物组。我们提供的证据表明，循环微生物组 DNA（cmDNA）受遗传和环境因素的驱动。更具体地说，我们发现循环微生物组特征是种特异性的，并且根据性别、大小、温度、条件因子和地理位置而变化。总的来说，我们的研究为检测野生鱼类种群中的生态失调特征和疾病风险提供了一种新方法，这对于渔业管理非常重要，尤其是在气候变化的背景下。
