School of Agriculture and Applied Sciences, Langston University, Langston, OK, 73050, USA.
Institute for Biosecurity and Microbial Forensics, Oklahoma State University, Stillwater, OK, 74078, USA.
Sci Rep. 2022 Mar 30;12(1):5362. doi: 10.1038/s41598-022-08939-x.
The Alpine goat (Capra aegagrus hircus) is parasitized by the barber pole worm (Haemonchus contortus). Hematological parameters from transcript and metagenome analysis in the host are reflective of infestation. We explored comparisons between blood samples of control, infected, infected zoledronic acid-treated, and infected antibody (anti-γδ T cells) treated wethers under controlled conditions. Seven days post-inoculation (dpi), we identified 7,627 transcripts associated with the different treatment types. Microbiome measurements at 7 dpi revealed fewer raw read counts across all treatments and a less diverse microbial flora than at 21 dpi. This study identifies treatment specific transcripts and an increase in microflora abundance and diversity as wethers age. Further, F/B ratio reflect health, based on depression or elevation above thresholds defined by the baseline of non-infected controls. Forty Alpine wethers were studied where blood samples were collected from five goats in four treatment groups on 7 dpi and 21 dpi. Transcript and microbiome profiles were obtained using the Partek Flow (St. Louis, Missouri, USA) software suites pipelines. Inflammation comparisons were based on the Firmicutes/Bacteriodetes ratios that are calculated as well as the reduction of microbial diversity.
高山山羊（Capra aegagrus hircus）被旋毛线虫（Haemonchus contortus）寄生。宿主转录组和宏基因组分析的血液参数反映了感染情况。我们在受控条件下探索了对照、感染、感染唑来膦酸治疗和感染抗 γδ T 细胞治疗的绵羊血液样本之间的比较。在接种后 7 天（dpi），我们确定了与不同治疗类型相关的 7627 个转录本。7 dpi 的微生物组测量结果显示，与 21 dpi 相比，所有处理的原始读数计数较少，微生物菌群的多样性也较低。这项研究确定了治疗特异性转录本，随着绵羊年龄的增长，微生物菌群的丰度和多样性增加。此外，F/B 比反映了健康状况，基于抑郁或升高超过由非感染对照的基线定义的阈值。对 40 只高山绵羊进行了研究，在接种后 7 天和 21 天从四个治疗组的五只羊中采集了血液样本。使用 Partek Flow（密苏里州圣路易斯，美国）软件套件管道获得转录组和微生物组图谱。炎症比较基于计算的厚壁菌门/拟杆菌门比值以及微生物多样性的减少。
