Internal Medicine Department, Faculty of Medicine Kasr Al-Ainy Hospital, Cairo University, Cairo, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Genes Immun. 2023 Apr;24(2):108-115. doi: 10.1038/s41435-023-00200-3. Epub 2023 Apr 12.
The main aim of this study was to assess the expression level of circulating long non-coding RNA maternally expressed gene 3 (lncRNA-MEG3), microRNA (miR-125a-5P), the chemokine C-X-C motif ligand13 (CXCL13), and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) in immune thrombocytopenia (ITP) cases and to study its relation to the disease severity and treatment response. This case-control study included 45 patients newly diagnosed as ITP and 45 healthy subjects. We assessed complete blood count, antinuclear antibodies, hepatitis B and C virus serology, lncRNA-MEG3, miR-125a-5P, and CXCL13 expression in serum by real-time PCR and NF-kb protein by ELISA. In ITP patients compared to control, lncRNA-MEG3 was significantly increased, and miRNA-125a-5P was decreased, and this was associated with higher CXCL13 and NF-kB levels (P < 0.001, for all).There was a significant negative correlation between platelet count and lncRNA-MEG3, CXCL13, and NF-kb, while a positive correlation with miR-125a-5p in ITP patients. Patients who responded to steroids had significantly higher miR-125a-5p (P = 0.016) and significantly lower lncRNA-MEG3 (P < 0.001), CXCL13 (P = 0.005), and NF-kb (p = 0.002). Based on the ROC curves, lncRNA-MEG3 displayed the highest area under the curve (AUC) in the identification of organ bleeding (AUC = 0.805), the response to steroids (AUC = 0.853), and the need for splenectomy (AUC = 0.75).
本研究的主要目的是评估循环长非编码 RNA 母系表达基因 3（lncRNA-MEG3）、微小 RNA（miR-125a-5P）、趋化因子 C-X-C 基序配体 13（CXCL13）和核因子 kappa-轻链增强子的活化 B 细胞（NF-kB）在免疫性血小板减少症（ITP）病例中的表达水平，并研究其与疾病严重程度和治疗反应的关系。这项病例对照研究包括 45 例新诊断为 ITP 的患者和 45 例健康对照者。我们通过实时 PCR 评估全血细胞计数、抗核抗体、乙型肝炎和丙型肝炎病毒血清学、lncRNA-MEG3、miR-125a-5P 和血清中 CXCL13 表达，并通过 ELISA 评估 NF-kB 蛋白。与对照组相比，ITP 患者的 lncRNA-MEG3 显著增加，miR-125a-5P 减少，且与更高的 CXCL13 和 NF-kB 水平相关（均 P<0.001）。在 ITP 患者中，血小板计数与 lncRNA-MEG3、CXCL13 和 NF-kB 呈显著负相关，与 miR-125a-5p 呈显著正相关。对类固醇有反应的患者 miR-125a-5p 显著升高（P=0.016），lncRNA-MEG3（P<0.001）、CXCL13（P=0.005）和 NF-kB（P=0.002）显著降低。基于 ROC 曲线，lncRNA-MEG3 在识别器官出血（AUC=0.805）、对类固醇的反应（AUC=0.853）和脾切除术的需要（AUC=0.75）方面显示出最高的曲线下面积（AUC）。
