Shaker Olfat, Mahfouz Hala, Salama Ahmad, Medhat Engy
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Kafrelsheikh University, Egypt.
Rep Biochem Mol Biol. 2020 Jul;9(2):230-240. doi: 10.29252/rbmb.9.2.230.
We aimed to evaluate the effectiveness of Highly Upregulated in Liver Cancer (HULC) and microRNA-372 (miR-372) as biochemical markers in Hepatocellular carcinoma (HCC) and HCV-infected patients.
The present study was conducted on 100 Egyptian individuals divided into 3 groups, 40 patients with HCC and HCV infection, 40 patients only HCV-infected, and 20 individuals as normal controls. They were subject to full history taking, full clinical and laboratory examination, and assessment of HULC and miR-372 levels by real-time PCR.
A statistically significant difference was found with p< 0.05 between HCC and each of HCV and control groups as regards HULC level with high mean among HCC followed by HCV patients. Our results also show a statistically significant difference with p< 0.05 between each of HCC and HCV compared to control as regards miR-372 level with low mean among HCC patients.
HULC could be considered as a potential non-invasive marker for detection and early diagnosis of HCC. Also, it may play an important role in the early prophylaxis and control measures to reduce the incidence of HCC. However, miR-372 cannot be considered as a reliable marker as HULC for early detection of HCC especially in HCV patients.
我们旨在评估肝癌高表达基因（HULC）和微小RNA-372（miR-372）作为肝细胞癌（HCC）和丙型肝炎病毒（HCV）感染患者生化标志物的有效性。
本研究对100名埃及个体进行，分为3组，40例HCC合并HCV感染患者，40例仅HCV感染患者，20例个体作为正常对照。对他们进行全面的病史采集、全面的临床和实验室检查，并通过实时聚合酶链反应评估HULC和miR-372水平。
就HULC水平而言，HCC组与HCV组和对照组之间均存在统计学显著差异（p<0.05），HCC组的平均水平最高，其次是HCV患者。我们的结果还显示，就miR-372水平而言，HCC组和HCV组与对照组之间均存在统计学显著差异（p<0.05），HCC患者的平均水平较低。
HULC可被视为检测和早期诊断HCC的潜在非侵入性标志物。此外，它可能在早期预防和控制措施中发挥重要作用，以降低HCC的发病率。然而，miR-372不能被视为像HULC那样用于早期检测HCC的可靠标志物，尤其是在HCV患者中。
