Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture Rural Affairs, College of Biological Science and Resources Environment, Beijing University of Agriculture, HuilongguanBeinonglu 7, Changping District, Beijing, 102206, China.
Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, HuilongguanBeinonglu 7, Changping District, Beijing, 102206, China.
BMC Genomics. 2023 Apr 12;24(1):195. doi: 10.1186/s12864-023-09274-9.
In plants, RNA silencing is an important conserved mechanism to regulate gene expression and combat against abiotic and biotic stresses. Dicer-like (DCL) and Argonaute (AGO) proteins and RNA-dependent RNA polymerase (RDR) are the core elements involved in gene silencing and their gene families have been explored in many plants. However, these genes and their responses to stresses have not yet been well characterized in adzuki bean.
A total of 11 AGO, 7 DCL and 6 RDR proteins were identified, and phylogenetic analyses of these proteins showed that they clustered into six, four and four clades respectively. The expression patterns of these genes in susceptible or resistant adzuki bean cultivars challenged with drought, bean common mosaic virus and Podosphaera xanthii infections were further validated by quantitative RT-PCR. The different responses of these proteins under abiotic and biotic stresses indicated their specialized regulatory mechanisms.
In this study, 24 genes of the DCL, AGO and RDR gene families in adzuki bean were identified, and the sequence characterization, structure of the encoded proteins, evolutionary relationship with orthologues in other legumes and gene expression patterns under drought and biotic stresses were primarily explored, which enriched our understanding of these genes in adzuki bean. Our findings provide a foundation for the comparative genomic analyses of RNA silencing elements in legume plants and further new insights into the functional complexity of RNA silencing in the response to various stresses in adzuki bean.
在植物中，RNA 沉默是一种重要的保守机制，可用于调节基因表达并抵抗非生物和生物胁迫。Dicer-like (DCL) 和 Argonaute (AGO) 蛋白以及 RNA 依赖性 RNA 聚合酶 (RDR) 是参与基因沉默的核心元件，它们的基因家族已在许多植物中得到了探索。然而，这些基因及其对胁迫的反应在红小豆中尚未得到很好的描述。
共鉴定出 11 种 AGO、7 种 DCL 和 6 种 RDR 蛋白，这些蛋白的系统发育分析表明，它们分别聚类为六个、四个和四个分支。通过定量 RT-PCR 进一步验证了这些基因在易感或抗性红小豆品种受到干旱、豆科普通花叶病毒和 Podosphaera xanthii 感染时的表达模式。这些蛋白在非生物和生物胁迫下的不同反应表明它们具有专门的调节机制。
本研究鉴定了红小豆 DCL、AGO 和 RDR 基因家族的 24 个基因，并初步探讨了这些基因的序列特征、编码蛋白的结构、与其他豆科植物同源物的进化关系以及在干旱和生物胁迫下的基因表达模式，丰富了我们对红小豆中这些基因的认识。我们的研究结果为豆科植物 RNA 沉默元件的比较基因组分析提供了基础，并进一步深入了解红小豆中 RNA 沉默在应对各种胁迫中的功能复杂性。
