Zhang Runqi, Shui Bonian, Jin Zihui, Cui Chenxi, Zhao Shiqi
School of Fishery, Zhejiang Ocean University, Zhoushan, 316022, China.
Zhejiang Marine Fisheries Research Institute, Zhoushan, 316021, China.
Biochem Biophys Rep. 2025 Aug 2;43:102197. doi: 10.1016/j.bbrep.2025.102197. eCollection 2025 Sep.
The AGO, DCL and RDR gene families work together in the plant gene silencing process, playing crucial roles in regulating gene expression, responding to stress, and gene silencing, thus forming an essential part of the plant's defense system. In this study, we systematically identified and characterized these genes in the closely related mangrove species and . We found a total of 8/6 AGO, 4/3 DCL and 5/4 RDR genes in these species, respectively. Phylogenetic analysis classified them into 3, 4, and 4 subfamilies correspondingly. Comparing with the sister species helped clarify key genes involved in cold tolerance. Each gene exhibited different expression levels across various tissues and stress conditions, with certain genes showing notably high expression. Furthermore, analyses of promoters, protein interaction networks, and three-dimensional structures provided deeper insights into the possible reasons behind the greater cold tolerance of compared to . These comprehensive results offer a theoretical basis for further experimental validation of the specific roles of these genes in 's stress resistance and for investigating gene-environment interactions.
AGO、DCL和RDR基因家族在植物基因沉默过程中协同作用，在调节基因表达、应对胁迫和基因沉默方面发挥关键作用，从而构成植物防御系统的重要组成部分。在本研究中，我们系统地鉴定并表征了亲缘关系相近的红树林物种[物种名称1]和[物种名称2]中的这些基因。我们在这些物种中分别共发现了8/6个AGO基因、4/3个DCL基因和5/4个RDR基因。系统发育分析将它们相应地分为3个、4个和4个亚家族。与姊妹物种[物种名称3]进行比较有助于明确参与耐寒性的关键基因。每个基因在不同组织和胁迫条件下表现出不同的表达水平，某些基因表现出显著的高表达。此外，对启动子、蛋白质相互作用网络和三维结构的分析为[物种名称1]比[物种名称2]具有更强耐寒性的可能原因提供了更深入的见解。这些综合结果为进一步实验验证这些基因在[物种名称1]抗逆性中的具体作用以及研究基因与环境相互作用提供了理论基础。
