National Oil Crops Improvement Center in Hunan, Department of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2023 Mar 30;24(7):6472. doi: 10.3390/ijms24076472.
The color of rapeseed ( L.) petal is usually yellow but can be milky-white to orange or pink. Thus, the petal color is a popular target in rapeseed breeding programs. In his study, metabolites and RNA were extracted from the yellow (Y), yellow/purple (YP), light purple (LP), and purple (P) rapeseed petals. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), RNA-Seq, and quantitative real-time (qRT-PCR) analyses were performed to analyze the expression correlation of differential metabolites and differential genes. A total of 223 metabolites were identified in the petals of the three purple and yellow rapeseed varieties by UPLC-MS/MS. A total of 20511 differentially expressed genes (DEGs) between P, LP, YP, versus Y plant petals were detected. This study focused on the co-regulation of 4898 differential genes in the three comparison groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation and quantitative RT-PCR analysis showed that the expression of () affects the synthesis of downstream peonidin and delphinidin and is a key gene regulating the purple color of petals in L. The gene may play a key role in regulating rapeseed flower color; however, further studies are needed to verify this. These results deepen our understanding of the molecular mechanisms underlying petal color and provide the theoretical and practical basis for flower breeding targeting petal color.
油菜花瓣的颜色通常为黄色，但也可能呈乳白色、橙色或粉红色。因此，花瓣颜色是油菜育种计划中的一个热门目标。在他的研究中，从黄色（Y）、黄/紫（YP）、浅紫色（LP）和紫色（P）油菜花瓣中提取了代谢物和 RNA。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）、RNA-Seq 和定量实时（qRT-PCR）分析，分析差异代谢物和差异基因的表达相关性。通过 UPLC-MS/MS 在三种紫色和黄色油菜品种的花瓣中鉴定出 223 种代谢物。在 P、LP、YP 与 Y 植株花瓣之间共检测到 20511 个差异表达基因（DEGs）。本研究重点关注四个比较组中 4898 个差异基因的共同调控。京都基因与基因组百科全书（KEGG）功能注释和定量 RT-PCR 分析表明，（）的表达影响下游矢车菊素和天竺葵素的合成，是调控油菜花瓣紫色的关键基因。该基因可能在调控油菜花色中起关键作用；然而，需要进一步的研究来验证这一点。这些结果加深了我们对花瓣颜色分子机制的理解，为花色育种提供了理论和实践基础。
