Cui Cheng, Zhang Ka, Chai Liang, Zheng Benchuan, Zhang Jinfang, Jiang Jun, Tan Chen, Li Haojie, Chen Daozong, Jiang Liangcai
Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
College of Life Sciences, Ganzhou Key Laboratory of Greenhouse Vegetable, Gannan Normal University, Ganzhou, China.
Front Plant Sci. 2024 Jun 12;15:1419508. doi: 10.3389/fpls.2024.1419508. eCollection 2024.
is one of the most important oil crops in the world. Breeding oilseed rape with colorful flowers can greatly enhance the ornamental value of and thus improve the economic benefits of planting. As water-soluble flavonoid secondary metabolites, anthocyanins are very important for the synthesis and accumulation of pigments in the petals of plants, giving them a wide range of bright colors. Despite the documentation of over 60 distinct flower shades in , the intricacies underlying flower color variation remain elusive. Particularly, the mechanisms driving color development across varying flower color backgrounds necessitate further comprehensive investigation. This research undertook a comprehensive exploration through the integration of transcriptome and metabolome analyses to pinpoint pivotal genes and metabolites underpinning an array of flower colors, including beige, beige-red, yellow, orange-red, deep orange-red, white, light-purple, and purple. First, we used a two-way BLAST search to find 275 genes in the reference genome of Darmor v10 that were involved in making anthocyanins. The subsequent scrutiny of RNA-seq outcomes underscored notable upregulation in the structural genes and , alongside the , , and transcriptional regulators within petals, showing anthocyanin accumulation. By synergizing this data with a weighted gene co-expression network analysis, we identified , , , , and as the key players driving anthocyanin synthesis in beige-red, orange-red, deep orange-red, light-purple, and purple petals. By integrating transcriptome and weighted gene co-expression network analysis findings with anthocyanin metabolism data, it is hypothesized that the upregulation of , which, in turn, enhances expression, plays a pivotal role in the development of pigmented oilseed rape flowers. These findings help to understand the transcriptional regulation of anthocyanin biosynthesis in and provide valuable genetic resources for breeding varieties with novel flower colors.
是世界上最重要的油料作物之一。培育具有彩色花朵的油菜可以大大提高其观赏价值，从而提高种植的经济效益。作为水溶性黄酮类次生代谢产物，花青素对于植物花瓣中色素的合成和积累非常重要，赋予它们广泛的鲜艳颜色。尽管已记录了油菜超过60种不同的花色，但花色变异背后的复杂机制仍然难以捉摸。特别是，驱动不同花色背景下颜色发育的机制需要进一步全面研究。本研究通过整合转录组和代谢组分析进行了全面探索，以确定一系列花色（包括米色、米红色、黄色、橙红色、深橙红色、白色、浅紫色和紫色）背后的关键基因和代谢物。首先，我们通过双向BLAST搜索在油菜Darmor v10参考基因组中找到了275个参与花青素合成的基因。随后对RNA-seq结果的仔细检查强调了花瓣中结构基因和以及转录调节因子的显著上调，表明花青素积累。通过将这些数据与加权基因共表达网络分析相结合，我们确定、、、和是驱动米红色、橙红色、深橙红色、浅紫色和紫色花瓣中花青素合成的关键因素。通过将转录组和加权基因共表达网络分析结果与花青素代谢数据相结合，推测的上调进而增强的表达，在有色油菜花朵的发育中起关键作用。这些发现有助于了解油菜中花青素生物合成的转录调控，并为培育具有新颖花色的油菜品种提供有价值的遗传资源。
