Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2023 Mar 31;24(7):6574. doi: 10.3390/ijms24076574.
, a novel type of mysterious DNA transposons discovered computationally prior to bench work confirmation, are components ubiquitous in most sequenced genomes of various eukaryotes, including plants, animals, and fungi. There is a paucity of empirical evidence to elucidate the mechanism of transposition in plants. Here, by constructing several artificial defective () reporter systems, we aim to identify the autonomous () in maize genetically and to demonstrate the transposition and repair mechanisms of upon the excision in maize. When crossing with various inbred lines, several transgenic lines produced progeny of segregated, purple-blotched kernels, resulting from a leaky expression of the gene driven by the -interrupted promoter. Transcription analysis indicated that the insertion of different into the promoter or exon would lead to multiple distinct mRNA transcripts corresponding to transgenes in the host genome. Simple excision products and circular intermediates of transposition have been detected from the leaf tissue of the seedlings in F hybrids of transgenic lines with corresponding tester, although they failed to be detected in all primary transgenic lines. These results revealed the transposition and repair mechanism of in maize. It is strongly suggested that this reporter system can detect the genetic activity of autonomic at the molecular level. Sequence features of itself, together with the flanking regions, impact the excision activity of and the regulation of the on the transcription level of the host gene.
, 一种新型的神秘 DNA 转座子，在实验工作确认之前通过计算发现，是普遍存在于各种真核生物（包括植物、动物和真菌）的大多数已测序基因组中的成分。目前，缺乏实证证据来阐明植物中转座的机制。在这里，我们通过构建几个人工缺陷 () 报告系统，旨在从遗传学上鉴定玉米中的自主 ()，并展示玉米中 的转座和修复机制。当与各种自交系杂交时，几个转基因系产生了分离的、紫色斑点的籽粒后代，这是由于 - 中断启动子驱动的 基因的渗漏表达。转录分析表明，不同 插入到 启动子或外显子中，会导致宿主基因组中转基因产生多个不同的 mRNA 转录本。虽然在所有初级转基因系中都未能检测到，但从转基因系与相应 测试者的 F 杂种幼苗的叶片组织中检测到了 转座的简单切除产物和环状中间产物。这些结果揭示了 在玉米中的转座和修复机制。强烈建议该报告系统可以在分子水平上检测自主 的遗传活性。 本身的序列特征以及侧翼区域，影响 的切除活性和 对宿主基因转录水平的调控。
