Hsu Wei-Tse, Williamson Peter, Khatkar Mehar Singh
Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Sydney, NSW 2006, Australia.
School of Animal and Veterinary Sciences, Faculty of Sciences, Engineering and Technology, The University of Adelaide, Roseworthy, SA 5371, Australia.
Animals (Basel). 2023 Mar 24;13(7):1149. doi: 10.3390/ani13071149.
Dog breeds represent canine sub-populations with distinctive phenotypic features and limited genetic diversity. We have established a resource to study breed-specific genetic diversity. Utilising genetic resources within our laboratory biobank, public domain genotype data and the phylogenetic framework of 23 breed clades, the primary objective for this study was to identify genomic regions that differentiate the Bullmastiff breed. Through application of a composite index analysis (CSS), genomic signatures were identified in Bullmastiffs when compared to the formative breeds, Mastiffs and Bulldogs, and to 22 other breed groups. Significant regions were identified on 15 chromosomes, with the most differentiated regions found on CFA1, CFA9, and CFA18. These regions may reflect genetic drift following establishment of the breed or the effects of selective breeding during development of the modern Bullmastiff. This was supported by analysis of genes from the identified genomic regions, including 458 genes from the multi-clade analysis, which revealed enriched pathways that may be related to characteristic traits and distinct morphology of the breed. The study demonstrates the utility of the CSS method in breed-specific genome analysis and advances our understanding of genetic diversity in Bullmastiff dogs.
犬种代表了具有独特表型特征和有限遗传多样性的犬亚群。我们建立了一个资源库来研究特定品种的遗传多样性。利用我们实验室生物样本库中的遗传资源、公共领域的基因型数据以及23个品种分支的系统发育框架，本研究的主要目的是确定区分斗牛獒犬品种的基因组区域。通过应用复合指数分析（CSS），与形成性品种马士提夫犬和斗牛犬以及其他22个品种组相比，在斗牛獒犬中鉴定出了基因组特征。在15条染色体上鉴定出了显著区域，其中在犬1号染色体（CFA1）、犬9号染色体（CFA9）和犬18号染色体（CFA18）上发现了差异最大的区域。这些区域可能反映了该品种建立后的遗传漂变或现代斗牛獒犬发育过程中选择性育种的影响。对来自已鉴定基因组区域的基因进行分析支持了这一点，包括来自多分支分析的458个基因，这些基因揭示了可能与该品种的特征性状和独特形态相关的富集途径。该研究证明了CSS方法在特定品种基因组分析中的实用性，并推进了我们对斗牛獒犬遗传多样性的理解。
