Biomedical Sciences Program, Midwestern University, Glendale, AZ 85308, USA.
College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Nutrients. 2023 Mar 23;15(7):1556. doi: 10.3390/nu15071556.
Maternal dietary levels of one-carbon (1C) metabolites (folic acid and choline) during pregnancy play a vital role in neurodevelopment. However, the impact of maternal dietary deficiencies on offspring stroke outcomes later in life remains undefined. The aim of this study was to investigate the role of maternal dietary deficiencies in folic acid and choline on ischemic stroke outcomes in middle-aged offspring. Female mice were maintained on either a control or deficient diet prior to and during pregnancy and lactation. At 10 months of age ischemic stroke was induced in male and female offspring. Stroke outcome was assessed by measuring motor function and brain tissue. There was no difference in offspring motor function; however, sex differences were present. In brain tissue, maternal dietary deficiency increased ischemic damage volume and offspring from deficient mothers had reduced neurodegeneration and neuroinflammation within the ischemic region. Furthermore, there were changes in plasma 1C metabolites as a result of maternal diet and sex. Our data indicate that maternal dietary deficiencies do not impact offspring behavior after ischemic stroke but do play a role in brain histology and one-carbon metabolite levels in plasma. Additionally, this study demonstrates that the sex of mice plays an important role in stroke outcomes.
母体在怀孕期间的一碳（1C）代谢物（叶酸和胆碱）的饮食水平对神经发育起着至关重要的作用。然而，母体饮食缺乏对后代生命后期中风结果的影响尚不清楚。本研究旨在探讨母体叶酸和胆碱饮食缺乏对中年后代缺血性中风结果的影响。雌性小鼠在怀孕和哺乳期前和期间分别维持在对照或缺乏饮食中。在 10 个月大时，雄性和雌性后代诱导缺血性中风。通过测量运动功能和脑组织来评估中风结果。后代的运动功能没有差异，但存在性别差异。在脑组织中，母体饮食缺乏增加了缺血性损伤体积，并且来自缺乏母亲的后代在缺血区域的神经退行性变和神经炎症减少。此外，由于母体饮食和性别，血浆中的 1C 代谢物发生了变化。我们的数据表明，母体饮食缺乏不会影响缺血性中风后后代的行为，但确实会影响脑组织学和血浆中一碳代谢物水平。此外，本研究表明，小鼠的性别在中风结果中起着重要作用。
