Department of Biomedical Sciences, Midwestern University; Department of Basic Medical Sciences, College of Medicine Phoenix, University of Arizona; Department of Anesthesiology, Pharmacology, & Therapeutics, Faculty of Medicine, University of British Columbia.
Center of Metabolomics, Institute of Metabolic Disease, Baylor Scott & White Research Institute.
J Vis Exp. 2024 Jun 28(208). doi: 10.3791/66827.
Maternal nutrition during pregnancy and lactation plays an important role in the neurodevelopment of offspring. One-carbon (1C) metabolism, which centers around folic acid and choline, as well as other B vitamins, plays a key role during the closure of the neural tube of the developing fetus. However, the impact of these maternal nutritional deficiencies during pregnancy on offspring health outcomes after birth remains relatively undefined. Furthermore, maternal dietary deficiencies in folic acid or choline may impact other health outcomes in offspring - making this a valuable model. This protocol aims to outline the procedure for inducing a deficiency in 1C metabolism in female mice through dietary modifications. Females are placed on diets at weaning, up to 2 months of age, for 4-6 weeks prior to mating and remain on diet throughout pregnancy and lactation. Offspring from these females can be evaluated for health outcomes. Females can be used multiple times to generate offspring, and tissues from females can be collected to measure for 1C metabolite measurements. This protocol provides an overview of how to induce maternal dietary deficiencies in folic acid or choline to study offspring health outcomes.
孕期和哺乳期的母体营养对后代的神经发育起着重要作用。一碳（1C）代谢以叶酸和胆碱以及其他 B 族维生素为中心，在发育中胎儿神经管闭合过程中起着关键作用。然而，这些孕期母体营养缺乏对出生后后代健康结果的影响仍相对不明确。此外，母体叶酸或胆碱的饮食缺乏可能会影响后代的其他健康结果——这使其成为一个有价值的模型。本方案旨在概述通过饮食改变诱导雌性小鼠 1C 代谢缺乏的程序。雌性小鼠在断奶后开始饮食限制，持续 4-6 周，直至交配前，并在整个孕期和哺乳期保持饮食限制。这些雌性的后代可以评估健康结果。雌性可以多次使用以产生后代，并且可以从雌性中收集组织以测量 1C 代谢物测量值。本方案提供了诱导母体叶酸或胆碱饮食缺乏以研究后代健康结果的概述。
