Departamento de Química, Universidad Técnica Particular de Loja, Loja 110150, Ecuador.
Molecules. 2023 Mar 24;28(7):2910. doi: 10.3390/molecules28072910.
The species , commonly known as wax laurel, is a tree belonging to the Myricaceae family that can be found from Costa Rica to Bolivia. In this study, the chemical composition, enantiomeric distribution, and biological activity of essential oil isolated from the leaves of this species was determined. Hydrodistillation was used to isolate the essential oil (EO). Gas chromatography coupled with mass spectrometry was used to determine the qualitative composition, gas chromatography equipped with a flame ionization detector was used to determine quantitative composition, and gas chromatography on an enantioselective column was used to determine enantiomeric distribution. The broth microdilution method was employed to assess the antibacterial capacity of the essential oil against seven opportunistic microorganisms, including three Gram-positive cocci bacteria, a Gram-positive bacilli bacterium and three Gram-negative bacilli bacteria. 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical cation and 2,2-diphenyl-1-picrylhydryl free radical were used as reagents to determine the antioxidant activity of essential oil. The spectrophotometric method was used to analyze the acetylcholinesterase inhibitory effect of the essential oil. The extraction method afforded a low yield of around 0.076 ± 0.008% (/). Fifty-eight chemical compounds, which represent 97.9% of the total composition, were identified in the essential oil. Sesquiterpene hydrocarbons were the most representative group with 24 compounds (67.8%). The principal constituents were ()-caryophyllene (27.5 ± 1.3%), limonene (11.8 ± 0.6%), -selinene (9.1 ± 0.2%), -selinene (8.0 ± 0.2%), selina-3,7(11)-diene (5.3 ± 0.2%) and germacrene B (5.0 ± 0.5%). Three pairs of enantiomers were identified in the essential oil of . Essential oil presented strong activity against the bacterium with an MIC of 250 μg/mL. The antioxidant activity of essential oil was very strong in the ABTS method with an SC of 46.4 ± 1.0 µg/mL and was strong in the DPPH method with an SC of 237.1 ± 1.8 µg/mL. Additionally, the essential oil reported strong anticholinesterase activity with an IC of 133.5 ± 1.06 µg/mL.
这种物种，通常被称为月桂树，是桃金娘科的一种树，从哥斯达黎加到玻利维亚都有分布。在这项研究中，确定了从该物种叶子中分离出的精油的化学成分、对映体分布和生物活性。用水蒸气蒸馏法分离精油（EO）。气相色谱-质谱联用仪用于确定定性组成，配备火焰电离检测器的气相色谱仪用于确定定量组成，手性柱气相色谱用于确定对映体分布。肉汤微量稀释法评估精油对七种机会性微生物的抗菌能力，包括三种革兰氏阳性球菌、一种革兰氏阳性杆菌和三种革兰氏阴性杆菌。2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子和 2,2-二苯基-1-苦基肼自由基被用作测定精油抗氧化活性的试剂。分光光度法用于分析精油对乙酰胆碱酯酶的抑制作用。提取方法的产率很低，约为 0.076±0.008% (/). 从精油中鉴定出 58 种化合物，占总成分的 97.9%。倍半萜类化合物是最具代表性的一类，有 24 种化合物（67.8%）。主要成分是()-丁香烯（27.5±1.3%）、柠檬烯（11.8±0.6%）、-selinene（9.1±0.2%）、-selinene（8.0±0.2%）、selina-3,7(11)-diene（5.3±0.2%）和倍半萜 B（5.0±0.5%）。从 中鉴定出三对对映体。精油对细菌表现出很强的活性，MIC 为 250μg/mL。精油在 ABTS 法中具有很强的抗氧化活性，SC 为 46.4±1.0µg/mL，在 DPPH 法中具有较强的抗氧化活性，SC 为 237.1±1.8µg/mL。此外，该精油还具有较强的抗胆碱酯酶活性，IC 为 133.5±1.06µg/mL。
