Salinas Melissa, Calva James, Cartuche Luis, Valarezo Eduardo, Armijos Chabaco
Maestría en Química Aplicada, Universidad Técnica Particular de Loja, San Cayetano s/n, Loja 1101608, Ecuador.
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, Loja 1101608, Ecuador.
Plants (Basel). 2022 Apr 28;11(9):1188. doi: 10.3390/plants11091188.
The aim of this study was to extract and identify the chemical compounds of Diplosthephium juniperinum essential oil (EO) from Ecuador and to assess its anticholinesterase and antioxidant properties. The EO chemical composition was determined by GC−MS. A total of 74 constituents of EO were identified, representing 97.27% in DB-5ms and 96.06% in HP-INNOWax of the total EO. The major constituents (>4.50%) identified were: α-pinene (21.52, 22.04%), geranyl acetate (10.54, 7.78%), silphiper-fol-5-ene (8.67, 7.38%), α-copaene (8.26, 8.18%), 7-epi-silphiperfol-5-ene (4.93, 5.95%), and germacrene D (4.91, 6.00%). Enantioselective analysis of the volatile fraction of D. juniperinum showed: (+)-α-pinene as a pure enantiomer and 5 pairs of enantiomeric compounds. Among them, (−)-β-Pinene and (−)-Germacrene D presented a high enantiomeric excess of 93.23 and 84.62%, respectively, while (−)-α-Thujene, (−)-Sabinene and (S)-4-Terpineol with a lower enantiomeric excess of 56.34, 47.84 and 43.11%, respectively. A moderate inhibitory effect was observed for Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE) enzymes with IC50 values of 67.20 ± 7.10 and 89.00 ± 9.90 µg/mL, respectively. A lower antioxidant potential was observed for the EO measured through DPPH and ABTS radical scavenging assays with SC50 values of 127.03 and >1000 µg/mL, respectively. To the best of our knowledge, this is the first report of the chemical composition, enantiomeric distribution and, anticholinesterase and antioxidant potential of the EO of D. juniperinum. As future perspective, further in-vivo studies could be conducted to confirm the anticholinesterase potential of the EO.
本研究的目的是提取并鉴定来自厄瓜多尔的杜松叶双柱苏 essential oil（EO）的化学成分，并评估其抗胆碱酯酶和抗氧化特性。通过气相色谱 - 质谱联用（GC - MS）测定了 EO 的化学成分。共鉴定出 EO 的 74 种成分，在 DB - 5ms 柱上占总 EO 的 97.27%，在 HP - INNOWax 柱上占 96.06%。鉴定出的主要成分（>4.50%）为：α - 蒎烯（21.52, 22.04%）、乙酸香叶酯（10.54, 7.78%）、硅叶蕨 - 5 - 烯（8.67, 7.38%）、α - 可巴烯（8.26, 8.18%）、7 - 表 - 硅叶蕨 - 5 - 烯（4.93, 5.95%）和吉马烯 D（4.91, 6.00%）。对杜松叶双柱苏挥发成分的对映体分析表明：（+）-α - 蒎烯为纯对映体，还有 5 对对映体化合物。其中，（ - ）-β - 蒎烯和（ - ）-吉马烯 D 的对映体过量分别高达 93.23%和 84.62%，而（ - ）-α - 侧柏烯、（ - ）-桧烯和（S）-4 - 松油醇的对映体过量较低，分别为 56.34%、47.84%和 43.11%。观察到对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）有中等抑制作用，IC50 值分别为 67.20 ± 7.10 和 89.00 ± 9.90 µg/mL。通过 DPPH 和 ABTS 自由基清除试验测定，EO 的抗氧化潜力较低，SC50 值分别为 127.03 和>1000 µg/mL。据我们所知，这是关于杜松叶双柱苏 EO 的化学成分、对映体分布以及抗胆碱酯酶和抗氧化潜力的首次报道。作为未来展望，可以进行进一步的体内研究以确认 EO 的抗胆碱酯酶潜力。
