Umegaki K, Ikeda M, Tomita T
Thromb Res. 1986 Feb 1;41(3):415-23. doi: 10.1016/0049-3848(86)90252-5.
Aggregation and secretion of washed platelets from stroke-prone spontaneously hypertensive rats(SHRSP) were greatly reduced by the development of hypertension in comparison with age-matched normotensive WKY platelets. In an attempt to clarify the mechanism of the defective functions, Ca2+ transport in platelets from SHRSP and WKY were studied. Changes of cytoplasmic free Ca2+ concentration ([Ca2+]i) were examined by using Quin 2. [Ca2+]i increase in response to thrombin(0.028 - 0.11 U/ml) was significantly delayed in SHRSP platelets compared with that of age-matched WKY platelets. The time(sec) to peak in [Ca2+]i was about two times longer in SHRSP platelets than in WKY platelets. [Ca2+]i levels at resting state were significantly lower in SHRSP platelets while there was no difference in maximal [Ca2+]i level in response to thrombin (0.031 - 0.125 U/ml) between the two strains. In addition thrombin-induced 45Ca2+ uptake was significantly delayed in SHRSP platelets. This delay of [Ca2+]i increase following thrombin stimulation might be associated with the hypofunctions of SHRSP platelets.
与年龄匹配的正常血压WKY大鼠的血小板相比，易中风自发性高血压大鼠（SHRSP）洗涤血小板的聚集和分泌因高血压的发展而大大降低。为了阐明功能缺陷的机制，研究了SHRSP和WKY大鼠血小板中的Ca2+转运。使用Quin 2检测细胞质游离Ca2+浓度（[Ca2+]i）的变化。与年龄匹配的WKY大鼠血小板相比，SHRSP大鼠血小板中凝血酶（0.028 - 0.11 U/ml）诱导的[Ca2+]i增加明显延迟。SHRSP大鼠血小板中[Ca2+]i达到峰值的时间（秒）比WKY大鼠血小板长约两倍。SHRSP大鼠血小板静息状态下的[Ca2+]i水平明显较低，而两种品系在凝血酶（0.031 - 0.125 U/ml）刺激下的最大[Ca2+]i水平没有差异。此外，凝血酶诱导的45Ca2+摄取在SHRSP大鼠血小板中明显延迟。凝血酶刺激后[Ca2+]i增加的延迟可能与SHRSP大鼠血小板的功能减退有关。
