He Tiannan, Fan Jianshuai, Jiao Gaozhen, Liu Yuhan, Zhang Qimeng, Luo Ning, Ahmad Bilal, Chen Qingxi, Wen Zhifeng
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Plants (Basel). 2023 Apr 3;12(7):1543. doi: 10.3390/plants12071543.
Plant chitinases (EC 3.2.1.14) are pathogenesis-related (PR) proteins and are well studied in many plant species. However, little is known about the genomic organization and expression of chitinase genes in strawberries (). Here, 23 genes were identified in the genome of strawberry () and divided into GH18 and GH19 subfamilies based on phylogenetic relationships. A detailed bioinformatics analysis of the genes was performed, including gene physicochemical properties, chromosomal location, exon-intron distribution, domain arrangement, and subcellular localization. Twenty-two genes showed upregulation after infection. Following the exogenous application of SA, , , and showed significant changes in expression. The ectopic expression of in increased resistance to via controlling the SA and JA signaling pathway genes (, , , and ). The FvChi-14 protein location was predicted in the cell wall or extracellular matrix. We speculate that is involved in disease resistance by regulating the SA and JA signaling pathways. The findings of this study provide a theoretical reference for the functional studies of genes and new candidates for strawberry stress resistance breeding programs.
植物几丁质酶（EC 3.2.1.14）是病程相关（PR）蛋白，在许多植物物种中都得到了充分研究。然而，关于草莓几丁质酶基因的基因组组织和表达情况却知之甚少。在此，在草莓基因组中鉴定出23个基因，并根据系统发育关系将其分为GH18和GH19亚家族。对这些基因进行了详细的生物信息学分析，包括基因理化性质、染色体定位、外显子-内含子分布、结构域排列和亚细胞定位。22个基因在感染后表现出上调。外源施加水杨酸后，[此处原文缺失具体基因名称]表现出显著的表达变化。[此处原文缺失具体基因名称]在[此处原文缺失具体植物名称]中的异位表达通过控制水杨酸和茉莉酸信号通路基因（[此处原文缺失具体基因名称]）增强了对[此处原文缺失具体病原体名称]的抗性。预测FvChi - 14蛋白定位于细胞壁或细胞外基质。我们推测[此处原文缺失具体基因名称]通过调节水杨酸和茉莉酸信号通路参与抗病过程。本研究结果为[此处原文缺失具体基因名称]的功能研究提供了理论参考，并为草莓抗逆育种计划提供了新的候选基因。
