利用 MEMS 力传感器研究 hiPSC-CMs 的跳动频率随温度的变化关系。
Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor.
机构信息
Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan.
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, Japan.
出版信息
Sensors (Basel). 2023 Mar 23;23(7):3370. doi: 10.3390/s23073370.
It is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CMs by integrating the temperature regulation system into our sensor platform, which can directly and quantitatively measure their mechanical motion. We measured the beating frequency of hiPSC-CMs at different environmental temperatures and found that the beating frequency increased as the temperature increased. Although the rate at which the beating frequency increased with temperature varied, the temperature at which the beating stopped was relatively stable at approximately 20 °C. The stopping of beating at this temperature was stable, even in immature hiPSC-CMs, and was considered to be a primitive property of cardiomyocytes.
预计人类诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）可用于治疗严重的心脏病。然而，人类成年心肌细胞和 hiPSC-CMs 的特性和功能不同，包括细胞成熟度。在这项研究中，我们通过将温度调节系统集成到我们的传感器平台中，重点研究了 hiPSC-CMs 的温度依赖性，该平台可以直接和定量地测量它们的机械运动。我们测量了不同环境温度下 hiPSC-CMs 的跳动频率，发现跳动频率随着温度的升高而增加。尽管跳动频率随温度升高的增加速率有所不同，但跳动停止的温度相对稳定，约为 20°C。在这个温度下停止跳动是稳定的，即使在未成熟的 hiPSC-CMs 中也是如此，这被认为是心肌细胞的原始特性。
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