Pozmogova I N, Khovrychev M P, Korolev P N
Mikrobiologiia. 1979 Jan-Feb;48(1):39-43.
The rates of incorporation of labelled precursors of protein (14C-DL-leucine) and RNA (14C-uracil) into the cells of synchronous yeast cultures of Pichia membranaefaciens, Hansenula anomala, Saccharomyces cerevisiae and Saccharomyces (fabospora) fragilis were studied at different temperatures. Synthesis of RNA and then of protein was inhibited in H. anomala if the temperature was increased above the optimal one. This is manifested even more distinctly in Sacch. cerevisiae and P. membranaefaciens. In the thermotolerant yeast Sacch. fragilis, the incorporation of 14C-uracil was inhibited at temperatures above 40 degrees C while the rate of protein synthesis did not decrease.
研究了在不同温度下,标记的蛋白质前体(14C-DL-亮氨酸)和RNA前体(14C-尿嘧啶)掺入膜醭毕赤酵母、异常汉逊酵母、酿酒酵母和脆壁酵母同步酵母培养细胞中的速率。如果温度升高到最佳温度以上,异常汉逊酵母中RNA的合成以及随后蛋白质的合成会受到抑制。在酿酒酵母和膜醭毕赤酵母中,这种现象表现得更为明显。在耐热酵母脆壁酵母中,40摄氏度以上的温度会抑制14C-尿嘧啶的掺入,而蛋白质合成速率并未降低。