Institute of Entomology, Guizhou University, Guizhou Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang 550025, China.
Qingzhen City Agricultural and Rural Bureau, Guiyang, Guizhou 551400, China.
J Insect Sci. 2023 Mar 1;23(2). doi: 10.1093/jisesa/iead020.
Western flower thrips (WFTs), Frankliniella occidentalis (Thysanoptera, Thripidae), is one of the most serious pests that attack rose flowers. Little is known about the effect of different parts of the rose flower on nutritional contents and digestive enzyme activities in thrips. This study assessed variations in the nutritional contents and digestive enzyme activities in the second-instar larvae and adults WFTs fed on 3 food types (rose petals, rose flowers, and honey solution + kidney bean pods) for multiple generations. The highest contents of soluble sugar (in 10% honey solution + kidney bean pods), amino acid (in rose flowers), and protein (in rose flowers) were observed, respectively. Soluble sugar and protein contents in the second-instar larvae and adults fed on rose petals decreased in the F1 generation but increased in the F2 generation and remained at higher levels until the F7 generation. Feeding of thrips with 3 food types increased the lipid content in the F1 generation, which peaked in the F2 generation and remained high until the F7 generation. In most cases, α-amylase and trypsin activities significantly decreased in the F1 generation after feeding on rose petals and then prominently increased in the F2 generation. In contrast, chymotrypsin activity remarkably increased and peaked in the F1 generation after second-instar larvae thrips fed on rose petals. There were correlations among the contents of 3 nutrient related positively with the activities of α-amylase and trypsin in WFTs second-instar larvae and adults, respectively. Overall, variations in the nutrient properties of the 3 food types caused changes in nutrient contents and digestive enzyme activities in thrips.
西方花蓟马（WFTs），Frankliniella occidentalis（缨翅目，蓟马科），是攻击玫瑰花的最严重害虫之一。对于玫瑰花的不同部位对蓟马营养成分和消化酶活性的影响知之甚少。本研究评估了第二代幼虫和成虫 WFTs 分别以 3 种食物类型（玫瑰花瓣、玫瑰花和蜂蜜溶液+豇豆荚）连续多代取食后的营养成分和消化酶活性的变化。在 10%蜂蜜溶液+豇豆荚中观察到最高的可溶糖（sugars）含量，在玫瑰花中观察到最高的氨基酸（amino acids）含量，在玫瑰花中观察到最高的蛋白质（protein）含量。在第一代中，以玫瑰花瓣为食的第二代幼虫和成虫的可溶糖和蛋白质含量降低，但在第二代中增加，并一直保持在较高水平，直到第七代。以 3 种食物类型饲养的蓟马增加了第一代的脂质含量，在第二代达到峰值，一直保持到第七代。在大多数情况下，以玫瑰花瓣为食的第二代幼虫和成虫的α-淀粉酶和胰蛋白酶活性在第一代中显著降低，然后在第二代中显著增加。相反，以玫瑰花瓣为食的第二代幼虫的 chymotrypsin 活性显著增加，并在第一代达到峰值。3 种与营养相关的营养成分含量之间存在正相关关系，分别与 WFTs 第二代幼虫和成虫的α-淀粉酶和胰蛋白酶活性相关。总体而言，3 种食物类型的营养特性变化导致了蓟马营养成分和消化酶活性的变化。
