Salih B A, Rosenbusch R F
Vet Microbiol. 1986 Feb;11(1-2):93-102. doi: 10.1016/0378-1135(86)90010-6.
An antiglobulin-ELISA has been developed to detect antibody activity to Mycoplasma bovoculi in sera, nasal fluids and lacrimal fluids of field and experimentally exposed calves. Low IgG activity with no IgM or IgA was detected in sera of experimental calves. In nasal and lacrimal fluids, IgA appeared as early as the first week following exposure to M. bovoculi and predominated in both of these fluids throughout the 9 wk observation period. Sera from field-exposed animals showed high IgG and IgM activities. The metabolic-inhibition (MI) test was applied to detect growth inhibition of M. bovoculi in those fluids. This property was found only in sera of exposed animals and thus could be used to test for M. bovoculi infection. The ELISA test and the MI test were considered reliable tests for the detection of antibodies to M. bovoculi infection. The implications of finding no growth-inhibiting activity in nasal and lacrimal fluids concurrent with a high IgA activity are discussed.
已开发出一种抗球蛋白酶联免疫吸附测定法（ELISA），用于检测田间和实验性暴露犊牛血清、鼻液和泪液中针对牛支原体的抗体活性。在实验犊牛的血清中检测到低IgG活性，未检测到IgM或IgA。在鼻液和泪液中，IgA早在暴露于牛支原体后的第一周就出现了，并且在整个9周的观察期内，这两种液体中IgA都占主导。来自田间暴露动物的血清显示出高IgG和IgM活性。采用代谢抑制（MI）试验检测这些液体中牛支原体的生长抑制情况。仅在暴露动物的血清中发现了这种特性，因此可用于检测牛支原体感染。ELISA试验和MI试验被认为是检测牛支原体感染抗体的可靠试验。文中讨论了在鼻液和泪液中未发现生长抑制活性但IgA活性较高的意义。
